Gong Haiyang, Shen Ping, Jin Li, Xing Changhong, Tang Fu
Capital Medical University Affiliated Beijing Tiantan Hospital, Beijing 100050, China.
Cancer Biother Radiopharm. 2005 Apr;20(2):155-62. doi: 10.1089/cbr.2005.20.155.
The aim of this study was to investigate the effects of Lycium barbarum polysaccharide (LBP) on irradiation- or chemotherapy-induced myelosuppressive mice and cultured peripheral blood mononuclear cells (PBMCs).
In an in vivo experiment, mice were irradiated with a sublethal dose of 550 cGy X-ray or intraperitoneally (i.p.) injected with carboplatin (CB) 125 mg/kg to produce severe myelosuppression. Four to 6 hours after the irradiation or injection, mice were subcutaneously (s.c.) injected with LBP (50, 100, and 200 mg/kg) daily from day 0 to day 6. Blood samples were collected from the tail veins of mice at different time points, and peripheral white blood cells (WBC), red blood cells (RBC), and platelet (PLT) counts were monitored. In an in vitro experiment, human PBMCs were incubated with LBP at different concentrations in combination with phytohemagglutinin (PHA), and the production of granulocyte colony-stimulating factor (G-CSF) was tested.
Compared to the control, 50 mg/kg LBP (LBP-L) significantly ameliorated the decrease of peripheral WBC of irradiated myelosuppressive mice on day 13, and 100 mg/kg LBP (LBP-M) did the same on days 17 and 21. All dosages of LBP significantly ameliorated the decrease of peripheral RBC of irradiated myelosuppressive mice on days 17 and 25. Two-hundred mg/kg LBP (LBP-H) and LBP-M significantly enhanced peripheral PLT counts of irradiated myelosuppressive mice on days 10, 13, 17, and 21, as did LBP-L on days 13 and 17. All dosages of LBP increased peripheral WBC counts of chemotherapy-induced myelosuppressive mice to some extent, but there was no statistic difference when compared to the control. LBP-H significantly ameliorated the decrease of peripheral RBC of chemotherapy-induced myelosuppressive mice on days 13, 15, 17, and 20, and LBP-M and LBP-L did the same on days 15 and 17. All dosages of LBP significantly enhanced peripheral PLT counts of chemotherapy-induced myelosuppressive mice on days 7 and 10, as did LBP-H on days 13, 15, and 17, and LBP-M on days 13 and 15. Also, LBP could obviously stimulate human PBMCs to produce G-CSF.
LBP promoted the peripheral blood recovery of irradiation or chemotherapy-induced myelosuppressive mice, and the effects may be the result of the stimulation of PBMCs to produce G-CSF.
本研究旨在探讨枸杞多糖（LBP）对辐射或化疗诱导的骨髓抑制小鼠及培养的外周血单个核细胞（PBMC）的影响。
在体内实验中，小鼠接受550 cGy的亚致死剂量X射线照射或腹腔注射125 mg/kg卡铂（CB）以产生严重骨髓抑制。照射或注射后4至6小时，从第0天至第6天，每天给小鼠皮下注射LBP（50、100和200 mg/kg）。在不同时间点从小鼠尾静脉采集血样，监测外周血白细胞（WBC）、红细胞（RBC）和血小板（PLT）计数。在体外实验中，将人PBMC与不同浓度的LBP联合植物血凝素（PHA）孵育，检测粒细胞集落刺激因子（G-CSF）的产生。
与对照组相比，50 mg/kg LBP（LBP-L）显著改善了辐射诱导的骨髓抑制小鼠在第13天外周血白细胞的减少，100 mg/kg LBP（LBP-M）在第17天和第21天有同样效果。所有剂量的LBP均显著改善了辐射诱导的骨髓抑制小鼠在第17天和第25天外周血红细胞的减少。200 mg/kg LBP（LBP-H）和LBP-M显著提高了辐射诱导的骨髓抑制小鼠在第10、13、17和21天的外周血小板计数，LBP-L在第13天和第17天也有同样效果。所有剂量的LBP在一定程度上增加了化疗诱导的骨髓抑制小鼠的外周血白细胞计数，但与对照组相比无统计学差异。LBP-H显著改善了化疗诱导的骨髓抑制小鼠在第13、15、17和20天外周血红细胞的减少，LBP-M和LBP-L在第15天和第17天有同样效果。所有剂量的LBP均显著提高了化疗诱导的骨髓抑制小鼠在第7天和第10天的外周血小板计数，LBP-H在第13、15和17天也有同样效果，LBP-M在第13天和第15天有同样效果。此外，LBP可明显刺激人PBMC产生G-CSF。
LBP促进了辐射或化疗诱导的骨髓抑制小鼠的外周血恢复，其作用可能是刺激PBMC产生G-CSF的结果。
