Zhou Jing, Pang Hua, Li Wenbo, Liu Qiong, Xu Lu, Liu Qian, Liu Ying
Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Biomed Res Int. 2016;2016:4147879. doi: 10.1155/2016/4147879. Epub 2016 May 26.
Lycium barbarum has been used for more than 2500 years as a traditional herb and food in China. We investigated the effects of Lycium barbarum polysaccharides (LBP) on apoptosis, oxidative damage, and expression of adhesion molecules in bone marrow mononuclear cells (BMNC) of mice injured by ionizing radiation. Kunming mice were exposed to X-rays; then mice in the LBP groups were continuously injected with various concentrations of LBP intraperitoneally for 14 days. Mice in the control group were continuously injected with normal saline (NS) by the same route for 14 days. A normal group was set up. After 1, 7, and 14 days of treatment, mice were killed and BMNC were extracted. Cell cycle, apoptosis, and the expression of adhesion molecules CD44 and CD49d were detected by flow cytometry. The levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were identified by colorimetric analyses. LBP significantly decreased the percentage of G0/G1 phase, apoptosis, MDA level, and expression of CD44 and CD49d and distinctly increased the activity of SOD. LBP showed a protective effect on BMNC against ionizing radiation-induced apoptosis and oxidative damage and altered the expression of adhesion molecule.
在中国，枸杞作为传统草药和食物已被使用了2500多年。我们研究了枸杞多糖（LBP）对受电离辐射损伤的小鼠骨髓单个核细胞（BMNC）凋亡、氧化损伤及黏附分子表达的影响。将昆明小鼠暴露于X射线下；然后LBP组小鼠连续14天腹腔注射不同浓度的LBP。对照组小鼠通过相同途径连续14天注射生理盐水（NS）。设立正常组。治疗1、7和14天后，处死小鼠并提取BMNC。通过流式细胞术检测细胞周期、凋亡及黏附分子CD44和CD49d的表达。通过比色分析确定丙二醛（MDA）和超氧化物歧化酶（SOD）的水平。LBP显著降低了G0/G1期百分比、凋亡率、MDA水平以及CD44和CD49d的表达，并明显提高了SOD活性。LBP对BMNC免受电离辐射诱导的凋亡和氧化损伤具有保护作用，并改变了黏附分子的表达。
