Jiao Kui, Wang Qing Xiang, Sun Wei, Jian Fang Fang
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
J Inorg Biochem. 2005 Jun;99(6):1369-75. doi: 10.1016/j.jinorgbio.2005.03.017.
A new bis(N-benzyl-benzotriazole)dichloro Co(II) complex (Co(bbt)(2)Cl(2)) was synthesized and the structure was characterized by X-ray crystallography, IR spectrum and elemental analysis. The electrochemical characterization of Co(bbt)(2)Cl(2) was measured in detail, and the interaction of this Co(II) complex with fish sperm DNA was studied by electrochemical techniques and ultraviolet-visible (UV-Vis) spectrophotometry. The cyclic voltammetry (CV) showed that Co(bbt)(2)Cl(2) had two reduction peaks and one oxidation peak on gold electrode. It was found that the currents of both the reduction peaks and the oxidation peak decreased significantly in the presence of DNA compared with those in the absence of DNA, which indicated that Co(bbt)(2)Cl(2) could interact with DNA. The binding of DNA with the complex was not only electrostatic binding but also intercalation.
合成了一种新型双（N-苄基苯并三唑）二氯钴（II）配合物（Co(bbt)(2)Cl(2)），并通过X射线晶体学、红外光谱和元素分析对其结构进行了表征。详细测定了Co(bbt)(2)Cl(2)的电化学性质，并采用电化学技术和紫外可见（UV-Vis）分光光度法研究了该钴（II）配合物与鱼精DNA的相互作用。循环伏安法（CV）表明，Co(bbt)(2)Cl(2)在金电极上有两个还原峰和一个氧化峰。研究发现，与不存在DNA时相比，在DNA存在的情况下，还原峰和氧化峰的电流均显著降低，这表明Co(bbt)(2)Cl(2)可以与DNA相互作用。DNA与该配合物的结合不仅是静电结合，还存在嵌入作用。
