Monteiro Siomara C, Matté Cristiane, Delwing Daniela, Wyse Angela T S
Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600 Anexo, CEP 90035-003 Porto Alegre, RS, Brazil.
Mol Cell Endocrinol. 2005 May 31;236(1-2):9-16. doi: 10.1016/j.mce.2005.03.006.
In the present work we investigated the effect of ovariectomy on Na+, K+-ATPase and acetylcholinesterase (AChE) activities in rat hippocampus. We also studied some parameters of oxidative stress, namely total radical-trapping antioxidant potential (TRAP), thiobarbituric acid-reactive substances (TBA-RS), as well as the antioxidant enzyme activities superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities. Our hypothesis is that ovariectomy might cause alterations in essential enzyme activities necessary to brain normal functioning and that these chances could be caused by oxidative stress. Female adult Wistar rats were divided into three groups: (1) naive (control); (2) sham-operated; and (3) ovariectomized. Thirty days after ovariectomy rats were sacrificed. Results showed that rats subjected to ovariectomy presented a significant increase in Na+, K+-ATPase, AChE and CAT activities, but did not change the oxidative stress parameters studied when compared to sham or naive rats. Since ovariectomy mimics postmenopausal changes, our findings showing alteration in the activities of brain Na+, K+-ATPase, AChE and CAT may be related to problems in postmenopausal women.
在本研究中，我们调查了卵巢切除对大鼠海马体中钠钾ATP酶（Na +，K + -ATPase）和乙酰胆碱酯酶（AChE）活性的影响。我们还研究了氧化应激的一些参数，即总自由基捕获抗氧化能力（TRAP）、硫代巴比妥酸反应性物质（TBA-RS），以及抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性。我们的假设是，卵巢切除可能会导致大脑正常功能所必需的关键酶活性发生改变，而这些变化可能是由氧化应激引起的。成年雌性Wistar大鼠被分为三组：（1）未处理组（对照组）；（2）假手术组；（3）卵巢切除组。卵巢切除术后30天处死大鼠。结果显示，与假手术组或未处理组大鼠相比，接受卵巢切除的大鼠的Na +，K + -ATPase、AChE和CAT活性显著增加，但所研究的氧化应激参数没有变化。由于卵巢切除模拟了绝经后的变化，我们发现大脑中Na +，K + -ATPase、AChE和CAT活性发生改变，这可能与绝经后女性的问题有关。
