Hjerling-Leffler Jens, Marmigère Frédéric, Heglind Mikael, Cederberg Anna, Koltzenburg Martin, Enerbäck Sven, Ernfors Patrik
Unit of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 77 Stockholm, Sweden.
Development. 2005 Jun;132(11):2623-32. doi: 10.1242/dev.01852. Epub 2005 May 4.
The boundary cap (BC) is a transient neural crest-derived group of cells located at the dorsal root entry zone (DREZ) that have been shown to differentiate into sensory neurons and glia in vivo. We find that when placed in culture, BC cells self-renew, show multipotency in clonal cultures and express neural crest stem cell (NCSCs) markers. Unlike sciatic nerve NCSCs, the BC-NCSC (bNCSCs) generates sensory neurons upon differentiation. The bNCSCs constitute a common source of cells for functionally diverse types of neurons, as a single bNCSC can give rise to several types of nociceptive and thermoreceptive sensory neurons. Our data suggests that BC cells comprise a source of multipotent sensory specified stem cells that persist throughout embryogenesis.
边界帽（BC）是一组短暂存在的、源自神经嵴的细胞，位于背根进入区（DREZ），已证实在体内可分化为感觉神经元和神经胶质细胞。我们发现，置于培养环境中时，BC细胞能够自我更新，在克隆培养中表现出多能性，并表达神经嵴干细胞（NCSC）标志物。与坐骨神经NCSC不同，BC-NCSC（bNCSC）在分化时会生成感觉神经元。bNCSC构成了功能多样的神经元类型的共同细胞来源，因为单个bNCSC可产生多种伤害性和温度感受性感觉神经元。我们的数据表明，BC细胞包含多能感觉特异性干细胞的来源，这些干细胞在整个胚胎发育过程中持续存在。
