Matsui Y, Toh-e A
Department of Biology, University of Tokyo, Japan.
Gene. 1992 May 1;114(1):43-9. doi: 10.1016/0378-1119(92)90705-t.
We have isolated two novel genes, designated as RHO3 (on chromosome IX) and RHO4 (on chromosome XI), belonging to the ras superfamily, from the yeast Saccharomyces cerevisiae. Deletion of RHO4 did not affect cell growth, but deletion of RHO3 caused a severe growth delay and a decrease in cell viability. The rho3 and rho4 disrupted genes displayed synthetic lethality at 30 degrees C. RHO3 and RHO4 seem to have overlapping functions in cell growth, because the growth defect in rho3 cells was suppressed by overexpression of RHO4 despite the low homology (about 35%) at the amino acid sequence level between the RHO3 and RHO4 proteins.
我们从酿酒酵母中分离出了两个新基因，分别命名为RHO3（位于九号染色体上）和RHO4（位于十一号染色体上），它们属于ras超家族。RHO4的缺失不影响细胞生长，但RHO3的缺失会导致严重的生长延迟和细胞活力下降。rho3和rho4缺失的基因在30摄氏度时表现出合成致死性。RHO3和RHO4似乎在细胞生长中具有重叠功能，因为尽管RHO3和RHO4蛋白在氨基酸序列水平上的同源性较低（约35%），但RHO4的过表达抑制了rho3细胞中的生长缺陷。
