Lee Yun-Ju, Pruzinsky Stephanie A, Braun Paul V
Department of Materials Science and Engineering, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Langmuir. 2004 Apr 13;20(8):3096-106.
A glucose-sensitive inverse opal hydrogel was synthesized through photopolymerization of 2-hydroxyethyl methacrylate and 3-acrylamidophenylboronic acid within the interstitial space of a dried poly(styrene) colloidal crystal template, followed by template removal. Charged complex formation between the phenylboronic acid functional group and the 1,2-cis-diol glucose resulted in reversible swelling of the inverse opal hydrogel, which was observed through shifts in the optical diffraction wavelength. The hydrogel was sensitive to glucose at physiological concentrations and ionic strength. The effects of phenylboronic acid concentration, ionic strength, and buffer pH on the equilibrium hydrogel swelling were also studied. The kinetics of hydrogel swelling was also examined, and it was found that the rate of diffraction shift matched well with diffusion-limited kinetics. Additionally, the diffraction response was compared with simulations using the scalar wave approximation and transfer matrix method.
通过在干燥的聚（苯乙烯）胶体晶体模板的间隙空间内对甲基丙烯酸2-羟乙酯和3-丙烯酰胺基苯硼酸进行光聚合反应，随后去除模板，合成了一种对葡萄糖敏感的反蛋白石水凝胶。苯基硼酸官能团与1,2-顺式二醇葡萄糖之间形成带电络合物，导致反蛋白石水凝胶发生可逆溶胀，这通过光学衍射波长的变化得以观察。该水凝胶在生理浓度和离子强度下对葡萄糖敏感。还研究了苯基硼酸浓度、离子强度和缓冲液pH对水凝胶平衡溶胀的影响。同时考察了水凝胶溶胀的动力学，发现衍射位移速率与扩散受限动力学匹配良好。此外，将衍射响应与使用标量波近似和转移矩阵方法的模拟结果进行了比较。
