Shi Gongpu, Si Luying, Cai Jinyang, Jiang Hao, Liu Yun, Luo Wei, Ma Huiru, Guan Jianguo
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, International School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, China.
School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, China.
Nanomaterials (Basel). 2024 Jun 1;14(11):964. doi: 10.3390/nano14110964.
Diabetes is a common disease that seriously endangers human health. Continuous glucose monitoring (CGM) is important for the prevention and treatment of diabetes. Glucose-sensing photonic nanochains (PNCs) have the advantages of naked-eye colorimetric readouts, short response time and noninvasive detection of diabetes, showing immense potential in CGM systems. However, the developed PNCs cannot disperse in physiological environment at the pH of 7.4 because of their poor hydrophilicity. In this study, we report a new kind of PNCs that can continuously and reversibly detect the concentration of glucose (C) in physiological environment at the pH of 7.4. Polyacrylic acid (PAA) added to the preparation of PNCs forms hydrogen bonds with polyvinylpyrrolidone (PVP) in FeO@PVP colloidal nanoparticles and the hydrophilic monomer -2-hydroxyethyl acrylamide (HEAAm), which increases the content of PHEAAm in the polymer shell of prepared PNCs. Moreover, 4-(2-acrylamidoethylcarbamoyl)-3-fluorophenylboronic acid (AFPBA), with a relatively low pKa value, is used as the glucose-sensing monomer to further improve the hydrophilicity and glucose-sensing performances of PNCs. The obtained FeO@(PVP-PAA)@poly(AFPBA-co-HEAAm) PNCs disperse in artificial serum and change color from yellow-green to red when C increases from 3.9 mM to 11.4 mM, showing application potential for straightforward CGM.
糖尿病是一种严重危害人类健康的常见疾病。连续血糖监测（CGM）对于糖尿病的预防和治疗至关重要。葡萄糖传感光子纳米链（PNCs）具有肉眼比色读数、响应时间短以及对糖尿病进行无创检测等优点，在CGM系统中显示出巨大潜力。然而，由于其亲水性差，已开发的PNCs在pH值为7.4的生理环境中无法分散。在本研究中，我们报道了一种新型PNCs，它能够在pH值为7.4的生理环境中连续且可逆地检测葡萄糖（C）的浓度。添加到PNCs制备过程中的聚丙烯酸（PAA）与FeO@PVP胶体纳米颗粒中的聚乙烯吡咯烷酮（PVP）以及亲水性单体-2-羟乙基丙烯酰胺（HEAAm）形成氢键，这增加了制备的PNCs聚合物壳层中PHEAAm的含量。此外，具有相对较低pKa值的4-(2-丙烯酰胺基乙基氨基甲酰基)-3-氟苯硼酸（AFPBA）被用作葡萄糖传感单体，以进一步提高PNCs的亲水性和葡萄糖传感性能。所获得的FeO@(PVP-PAA)@聚(AFPBA-co-HEAAm) PNCs可分散在人工血清中，当C从3.9 mM增加到11.4 mM时，颜色从黄绿色变为红色，显示出用于直接CGM的应用潜力。
