Obee Peter C, Griffith Chris J, Cooper Rose A, Cooke Richard P, Bennion Nerys E, Lewis Michael
School of Applied Sciences, University of Wales Institute, Cardiff, United Kingdom.
Am J Infect Control. 2005 May;33(4):202-6. doi: 10.1016/j.ajic.2004.07.008.
To evaluate the efficacy of endoscope reprocessing using bacteriologic methods and adenosine triphosphate (ATP) bioluminescence and to evaluate the potential benefits of the latter in managing the process.
An endoscopy unit in each of 2 acute district general hospitals in the United Kingdom.
Following visual observation, 8 locations important in the decontamination of gastrointestinal (GI) endoscopes were monitored during reprocessing by bacteriologic sampling (paddle method) and ATP bioluminescence.
Endoscope reprocessing was conducted in accordance with British Society of Gastroenterology guidelines. Bacteriologic tests required 24 hours for results to be obtained; ATP tests were completed within 2 minutes. Of the 504 sites tested throughout the decontamination process, bacterial growth was recorded on 32 occasions, 3 from the end product. Throughout the decontamination process, ATP tests obtained 95 results above benchmark values and 1 from the end product.
The bacteriologic results after terminal disinfection indicated that the reprocessing was carried out effectively overall. ATP results prior to disinfection indicated that manual cleaning might not have always been performed adequately and that this could have impaired the disinfection process. ATP provided a rapid means of assessing the efficacy of cleaning steps prior to terminal disinfection.
采用细菌学方法和三磷酸腺苷（ATP）生物发光法评估内镜再处理的效果，并评估后者在管理该过程中的潜在益处。
英国两家急性区综合医院的内镜科室。
在目视观察后，通过细菌学采样（平板法）和ATP生物发光法对胃肠道（GI）内镜去污过程中8个重要部位进行监测。
内镜再处理按照英国胃肠病学会指南进行。细菌学检测需要24小时才能获得结果；ATP检测在2分钟内完成。在整个去污过程中检测的504个部位中，有32次记录到细菌生长，其中3次来自最终产品。在整个去污过程中，ATP检测有95次结果高于基准值，1次来自最终产品。
终末消毒后的细菌学结果表明，再处理总体上有效进行。消毒前的ATP结果表明，人工清洗可能并不总是充分进行，这可能会损害消毒过程。ATP提供了一种快速评估终末消毒前清洗步骤效果的方法。
