通过富血小板血浆法或白膜层法制备的血小板浓缩物中的白细胞碎片。
White blood cell fragments in platelet concentrates prepared by the platelet-rich plasma or buffy-coat methods.
作者信息
Dijkstra-Tiekstra M J, van der Schoot C E, Pietersz R N I, Reesink H W
机构信息
Sanquin Blood Bank North-west Region, 100 AC Amsterdam, the Netherlands.
出版信息
Vox Sang. 2005 May;88(4):275-7. doi: 10.1111/j.1423-0410.2005.00634.x.
BACKGROUND AND OBJECTIVES
White blood cell (WBC) fragments in platelet concentrates (PCs) may induce allo-immunization in the recipient.
MATERIALS AND METHODS
As the level of WBC fragments can differ between PCs produced using different methods, we compared PCs prepared by using the buffy-coat method (BC-PCs) in plasma or platelet additive solution (Composol) and PCs prepared using the platelet-rich plasma method (PRP-PCs).
RESULTS
Post-filtration results revealed identical levels of WBC, but significantly higher CD62p expression and a significantly lower amount of total DNA, cell-free DNA and number of WBC fragments (0.6 vs. 4.2 WBC equivalents/microl) in PRP-PCs than in BC-PCs in either plasma or Composol.
CONCLUSIONS
The number of WBC fragments is significantly higher in BC-PCs than in PRP-PCs.
背景与目的
血小板浓缩物（PCs）中的白细胞（WBC）碎片可能会诱导受者产生同种免疫。
材料与方法
由于使用不同方法制备的PCs中WBC碎片水平可能不同，我们比较了在血浆或血小板添加剂溶液（Composol）中使用白膜层法制备的PCs（BC-PCs）和使用富血小板血浆法制备的PCs（PRP-PCs）。
结果
过滤后结果显示，PRP-PCs中白细胞水平与BC-PCs相同，但PRP-PCs中CD62p表达显著更高，总DNA、游离DNA量及WBC碎片数量显著更低（0.6对4.2个白细胞当量/微升），无论是在血浆还是Composol中均如此。
结论
BC-PCs中WBC碎片数量显著高于PRP-PCs。
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