Dijkstra-Tiekstra M J, van der Schoot C E, Pietersz R N I, Reesink H W
Sanquin Blood Bank North-west Region, 100 AC Amsterdam, the Netherlands.
Vox Sang. 2005 May;88(4):275-7. doi: 10.1111/j.1423-0410.2005.00634.x.
White blood cell (WBC) fragments in platelet concentrates (PCs) may induce allo-immunization in the recipient.
As the level of WBC fragments can differ between PCs produced using different methods, we compared PCs prepared by using the buffy-coat method (BC-PCs) in plasma or platelet additive solution (Composol) and PCs prepared using the platelet-rich plasma method (PRP-PCs).
Post-filtration results revealed identical levels of WBC, but significantly higher CD62p expression and a significantly lower amount of total DNA, cell-free DNA and number of WBC fragments (0.6 vs. 4.2 WBC equivalents/microl) in PRP-PCs than in BC-PCs in either plasma or Composol.
The number of WBC fragments is significantly higher in BC-PCs than in PRP-PCs.
血小板浓缩物（PCs）中的白细胞（WBC）碎片可能会诱导受者产生同种免疫。
由于使用不同方法制备的PCs中WBC碎片水平可能不同，我们比较了在血浆或血小板添加剂溶液（Composol）中使用白膜层法制备的PCs（BC-PCs）和使用富血小板血浆法制备的PCs（PRP-PCs）。
过滤后结果显示，PRP-PCs中白细胞水平与BC-PCs相同，但PRP-PCs中CD62p表达显著更高，总DNA、游离DNA量及WBC碎片数量显著更低（0.6对4.2个白细胞当量/微升），无论是在血浆还是Composol中均如此。
BC-PCs中WBC碎片数量显著高于PRP-PCs。
