Department of Animal Medicine, Production and Health, University of Padova, Padova, Italy.
BMC Vet Res. 2013 Jul 22;9:146. doi: 10.1186/1746-6148-9-146.
To evaluate the efficiency of platelet-rich plasma preparations by means of a double centrifugation tube method to obtain platelet-rich canine plasma at a concentration at least 4 times higher than the baseline value and a concentration of white blood cells not exceeding twice the reference range. A complete blood count was carried out for each sample and each concentrate. Whole blood samples were collected from 12 clinically healthy dogs (consenting blood donors). Blood was processed by a double centrifugation tube method to obtain platelet concentrates, which were then analyzed by a flow cytometry haematology system for haemogram. Platelet concentration and white blood cell count were determined in all samples.
Platelet concentration at least 4 times higher than the baseline value and a white blood cell count not exceeding twice the reference range were obtained respectively in 10 cases out of 12 (83.3%) and 11 cases out of 12 (91.6%).
This double centrifugation tube method is a relatively simple and inexpensive method for obtaining platelet-rich canine plasma, potentially available for therapeutic use to improve the healing process.
通过双管离心法评估富血小板血浆制剂的效率，以获得至少比基线值高 4 倍的富犬血小板血浆和不超过参考范围两倍的白细胞浓度。对每个样本和每个浓缩物进行全血细胞计数。从 12 只临床健康犬（同意献血者）采集全血样本。通过双管离心法处理血液以获得血小板浓缩物，然后使用流式细胞术血液学系统对血液进行分析。在所有样本中均测定血小板浓度和白细胞计数。
在 12 例中有 10 例（83.3%）和 11 例（91.6%）分别获得至少比基线值高 4 倍的血小板浓度和不超过参考范围两倍的白细胞计数。
这种双管离心法是一种相对简单且经济的获取富犬血小板血浆的方法，可能可用于治疗以改善愈合过程。
