Karn Santosh Kumar, Pan Xiangliang, Jenkinson Ian R
Laboratory of Environmental Pollution and Bioremediation, Xinjiang Institute of Ecology and Geography, Chinese Academy of Science, South Beijing Road, Urumqi, Xinjiang, 830011, China.
Department of Biotechnology, National Institute of Technology, G.E.Road, Raipur, 492001, (CG), India.
3 Biotech. 2017 May;7(1):50. doi: 10.1007/s13205-017-0681-1. Epub 2017 Apr 25.
A combination of biological and chemical methods was applied in the present study to evaluate the removal of arsenic (As) from contaminated soil. The treatment involved As-oxidizing microbes aimed of transforming the more toxic As (III) to less toxic As (V) in the soil. FeCl was added at three different concentrations (1, 2, and 3%) to stabilize the As (V). Leaching of the treated soil was investigated by making a soil column and passing tap water through it to determine solubility. Experimental results indicated that the bacterial activity had a pronounced positive effect on the transformation of As, and decreased the soluble exchangeable fraction from 50 to 0.7 mg/kg as compared to control and from 50 to 44 mg/kg after 7 days of treatment. FeCl also played an indispensable role in the adsorption/stabilization of As in the soil; 1 and 2% FeCl strongly influenced the adsorption of As (V). The soil leachate contained negligible amount of As and trace metals, which indicates that combining an efficient microbe with a chemical treatment is very effective route for the removal and stabilization of As from contaminated soil in the environment.
本研究采用生物和化学方法相结合的方式，评估从受污染土壤中去除砷（As）的效果。该处理方法涉及利用砷氧化微生物将土壤中毒性更强的砷（III）转化为毒性较弱的砷（V）。添加了三种不同浓度（1%、2%和3%）的FeCl以稳定砷（V）。通过制作土柱并让自来水通过土柱来研究处理后土壤的淋溶情况，以确定其溶解度。实验结果表明，细菌活性对砷的转化有显著的积极影响，与对照相比，可溶交换态部分从50毫克/千克降至0.7毫克/千克，处理7天后从50毫克/千克降至44毫克/千克。FeCl在土壤中砷的吸附/稳定方面也发挥了不可或缺的作用；1%和2%的FeCl对砷（V）的吸附有强烈影响。土壤渗滤液中砷和痕量金属的含量可忽略不计，这表明将高效微生物与化学处理相结合是从环境中受污染土壤中去除和稳定砷的非常有效的途径。
