Duan Y R, Zhang Z R, Wang C Y, Chen J Y, Zhang X D
College of Material Science and Engineering, Dong Hua University, Shanghai 20051, China.
J Mater Sci Mater Med. 2004 Nov;15(11):1205-11. doi: 10.1007/s10856-004-5673-0.
Bone-like apatite formation on porous calcium phosphate ceramics was investigated in static simulated body fluid (SBF) and dynamic SBF at different flowing rates. The results of a 14-day immersion in static SBF showed that the formation of bone-like apatite occurred both on the surface and in the pores of the samples. When SBF flow at the physiological flow rate in muscle (2 ml/100 ml min1), bone-like apatite could be detected only in internal surface of the pores of samples. The result that bone-like apatite formation could only be found in the pores when SBF flown at physiological flow rate was consistent with that of porous calcium phosphate ceramics implanted in vivo: osteoinduction was only detected inside the pores of the porous calcium phosphate ceramics. This result implicates that the bone-like apatite may play an important role in the osteoinduction of Ca-P materials. The dynamic model used in this study may be better than usually used static immersion model in imitating the physiological condition of bone-like apatite formation. Dynamic SBF method is very useful to understand bone-like apatite formation in vivo and the mechanism of ectopic bone formation in calcium phosphate ceramics.
在不同流速的静态模拟体液（SBF）和动态SBF中研究了多孔磷酸钙陶瓷上类骨磷灰石的形成。在静态SBF中浸泡14天的结果表明，类骨磷灰石在样品表面和孔隙中均有形成。当SBF以肌肉中的生理流速（2 ml/100 ml min-1）流动时，仅在样品孔隙的内表面可检测到类骨磷灰石。当SBF以生理流速流动时，仅在孔隙中发现类骨磷灰石形成的结果与体内植入多孔磷酸钙陶瓷的结果一致：仅在多孔磷酸钙陶瓷的孔隙内检测到骨诱导作用。该结果表明类骨磷灰石可能在钙磷材料的骨诱导中起重要作用。本研究中使用的动态模型在模拟类骨磷灰石形成的生理条件方面可能优于通常使用的静态浸泡模型。动态SBF方法对于理解体内类骨磷灰石的形成以及磷酸钙陶瓷中异位骨形成的机制非常有用。
