Duan You-rong, Liu Ke-wei, Chen Ji-yong, Zhang Xing-dong
Space Med Med Eng (Beijing). 2002 Jun;15(3):203-7.
Objective. Bone-like apatite formation on the surface of calcium phosphate ceramics was believed to be the necessary step that new bone grows on the ceramics and to be relative to the osteoinductivity of the material. This study aimed at investigating the influence of the flow rate of simulated body fluid (SBF) (2 ml/min) in skeletal muscle upon the formation of bone-like apatite on porous calcium phosphate ceramics. Method. The dynamic condition was realized by controlling the SBF flowing in/out of the sample chamber of 100 ml. The flow rate of 2 ml/min is close to that in human muscle environment. The pH and inorganic ionic composition of SBF are close to those of human body fluid. Result. Bone-like apatite formation was relatively easier to occur in static SBF than in dynamic SBF. Experiment with flowing SBF (dynamic SBF) is better in mimicking the living body fluid than static SBF. Conclusion. The results from dynamic SBF may more truly show the relation between apatite layer formation and osteoinduction in biomaterials than that from in vitro experiments before.
目的。磷酸钙陶瓷表面骨样磷灰石的形成被认为是新骨在陶瓷上生长的必要步骤，且与材料的骨诱导性相关。本研究旨在探究骨骼肌中模拟体液（SBF）流速（2毫升/分钟）对多孔磷酸钙陶瓷表面骨样磷灰石形成的影响。方法。通过控制100毫升样品室中SBF的流入/流出实现动态条件。2毫升/分钟的流速接近人体肌肉环境中的流速。SBF的pH值和无机离子组成与人体体液相近。结果。与动态SBF相比，骨样磷灰石在静态SBF中相对更容易形成。流动SBF（动态SBF）实验在模拟活体体液方面比静态SBF更好。结论。动态SBF的结果可能比之前的体外实验结果更真实地显示生物材料中磷灰石层形成与骨诱导之间的关系。
