Duan Yourong, Wu Yao, Wang Chaoyuan, Chen Jiyong, Zhang Xingdong
Engineering Research Center in Biomaterials, Sichuan University, Chengdu 610064.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2003 Mar;20(1):22-5.
Bone-like apatite formation on the surface of calcium phosphate ceramics has been believed to be necessary for new bone to grow on the ceramics and to be related to the osteoinductivity of the material. The research of bone-like apatite formation is a great help to understanding the mechanism of osteoinduction. Synthetic porous calcium phosphate ceramics (HA/TCP = 70/30) were implanted intramuscularly in pigs, dogs, rabbits and rats to make a comparative study of the bone-like apatite formation onto the porous HA/TCP ceramics in different animals. Specimens were harvested at 14 days after implantation. Samples were detected for the surface morphology with SEM. The chemical composition of the sample surface after implantation was analyzed with reflection infrared (R-IR). Obvious bone-like apatite formation could be detected in the sections of porous specimens harvested from all animals after 14 days intramuscular implantation. Crystal deposition could be only observed on the surface of the concave regions of the samples collected from dogs, rabbits and rat. On the contrary, evenly distributed flake-shaped crystal could be found on the pore surface and also on the outer surface of the materials implanted in pigs. The morphology of bone-like apatite in pigs was different from that in the others animals. Bone-like apatite was not observed in dense specimen implanted intramuscularly. Bone-like apatite formed faster on specimens implanted in rabbit than that in other animals. This formation sequence is different from the sequence of osteoinductivity of biphasic calcium phosphate ceramics implanted in these animals. The results demonstrated that the formation of bone-like apatite on materials is a prerequisite condition to their osteoinduction but other factors also play important roles in osteoinduction.
人们认为，磷酸钙陶瓷表面形成类骨磷灰石是新骨在陶瓷上生长的必要条件，且与材料的骨诱导性有关。对类骨磷灰石形成的研究有助于理解骨诱导机制。将合成多孔磷酸钙陶瓷（HA/TCP = 70/30）肌肉内植入猪、狗、兔子和大鼠体内，对不同动物多孔HA/TCP陶瓷上类骨磷灰石的形成进行比较研究。植入后14天采集标本。用扫描电子显微镜（SEM）检测样品的表面形态。用反射红外光谱（R-IR）分析植入后样品表面的化学成分。肌肉内植入14天后，在所有动物采集的多孔标本切片中均可检测到明显的类骨磷灰石形成。仅在从狗、兔子和大鼠采集的样品凹面区域表面观察到晶体沉积。相反，在猪体内植入材料的孔隙表面和外表面均可发现均匀分布的片状晶体。猪体内类骨磷灰石的形态与其他动物不同。肌肉内植入的致密标本中未观察到类骨磷灰石。植入兔子体内的标本上类骨磷灰石的形成比其他动物更快。这种形成顺序与在这些动物体内植入双相磷酸钙陶瓷的骨诱导顺序不同。结果表明，材料上类骨磷灰石的形成是其骨诱导的前提条件，但其他因素在骨诱导中也起重要作用。
