Vierstraete Evy, Verleyen Peter, De Loof Arnold, Schoofs Liliane
Laboratory of Developmental Physiology, Genomics, and Proteomics, Katholieke Universiteit Leuven, Belgium.
Ann N Y Acad Sci. 2005 Apr;1040:504-7. doi: 10.1196/annals.1327.104.
Here, we report the identification of proteins associated with the immune response of Drosophila by analyzing the hemolymph profiles after infection. Two-dimensional difference gel electrophoresis was used to study the secretome in the hemolymph of Drosophila larvae. Shortly after induction with lipopolysaccharides, we identified 10 proteins, which we designated "Drosophila instantly released immune proteins". Infection with Micrococcus luteus or Saccharomyces cerevisiae induced 20 and 19 differential protein spots, respectively. Next to known immune proteins, new candidates that require further investigation were identified.
在此，我们通过分析感染后的血淋巴图谱报告了与果蝇免疫反应相关蛋白质的鉴定结果。二维差异凝胶电泳用于研究果蝇幼虫血淋巴中的分泌蛋白质组。在用脂多糖诱导后不久，我们鉴定出10种蛋白质，我们将其命名为“果蝇即时释放免疫蛋白”。用藤黄微球菌或酿酒酵母感染分别诱导出20个和19个差异蛋白点。除了已知的免疫蛋白外，还鉴定出了需要进一步研究的新候选蛋白。
