de Morais Guedes Sofia, Vitorino Rui, Domingues Rosário, Tomer Kenneth, Correia A J Ferrer, Amado Francisco, Domingues Pedro
Department of Chemistry, University of Aveiro, Portugal.
Biochem Biophys Res Commun. 2005 Mar 4;328(1):106-15. doi: 10.1016/j.bbrc.2004.12.135.
In the last decade, the fruit fly Drosophila melanogaster has emerged as a promising invertebrate model for the investigation of innate immunity, in part because of its well characterised genetics. The information provided by the innumerous reports on Drosophila's immune response indicates that a large number of genes, in addition to the well-known antimicrobial peptide genes, are both up- and down-regulated upon immune challenge. Nevertheless, their contribution to fighting off infection has not been seriously addressed. With the application of recent advances in proteomics, the effects of an immune challenge in the overall modification of Drosophila 2-DE protein patterns were investigated. The aim of this study was to investigate hemolymph proteins differentially expressed between control and immunised larvae sets, which could be related solely to the Drosophila immune response. The list of immune-related protein spots included heat shock proteins and other proteins with chaperone properties, serine proteases, phenol oxidase, and Drosophila antioxidant system components, which accounted for 21% of the total of 70 identified proteins, metabolic enzymes implicated in pathways such as cellular respiration, fatty-acid oxidation, protein biosynthesis, and structural proteins.
在过去十年中，果蝇已成为一种很有前景的用于研究先天免疫的无脊椎动物模型，部分原因是其遗传学特征明确。大量关于果蝇免疫反应的报告所提供的信息表明，除了众所周知的抗菌肽基因外，还有大量基因在免疫挑战后表达上调或下调。然而，它们在抵御感染中的作用尚未得到认真研究。随着蛋白质组学最新进展的应用，研究了免疫挑战对果蝇二维电泳蛋白质图谱整体修饰的影响。本研究的目的是调查对照幼虫组和免疫幼虫组之间差异表达的血淋巴蛋白，这些蛋白可能仅与果蝇的免疫反应有关。与免疫相关的蛋白质斑点列表包括热休克蛋白和其他具有伴侣性质的蛋白质、丝氨酸蛋白酶、酚氧化酶以及果蝇抗氧化系统成分，它们占已鉴定的70种蛋白质总数的21%，还包括参与细胞呼吸、脂肪酸氧化、蛋白质生物合成等途径的代谢酶以及结构蛋白。
