Vierstraete Evy, Verleyen Peter, Sas Filip, Van den Bergh Gert, De Loof Arnold, Arckens Lutgarde, Schoofs Liliane
Laboratory of Developmental Physiology, Genomics and Proteomics, K.U.Leuven, Naamsestraat 59, B-3000 Louvain, Belgium.
Biochem Biophys Res Commun. 2004 May 14;317(4):1052-60. doi: 10.1016/j.bbrc.2004.03.150.
In this study, we analyzed the hemolymph proteome of Drosophila third instar larvae, which were induced with a suspension of Gram-positive bacteria or yeast. Profiling of the hemolymph proteins of infected versus non-infected larvae was performed by two-dimensional difference gel electrophoresis. Infection with Micrococcus luteus or Saccharomyces cerevisiae induced, respectively, 20 and 19 differential protein spots. The majority of the spots are specifically regulated by one pathogen, whereas only a few spots correspond to proteins altered in all cases of challenging (including after challenge with lipopolysaccharides). All of the upregulated proteins can be assigned to specific aspects of the immune system, as they did not increase in the hemolymph of sterile pricked larvae. Next to known immune proteins, unannotated proteins were identified such as CG4306 protein, which has homologues with unknown function in all metazoan genome databases available today.
在本研究中，我们分析了经革兰氏阳性菌或酵母悬浮液诱导的果蝇三龄幼虫的血淋巴蛋白质组。通过二维差异凝胶电泳对感染幼虫与未感染幼虫的血淋巴蛋白质进行分析。藤黄微球菌或酿酒酵母感染分别诱导出20个和19个差异蛋白点。大多数斑点受一种病原体特异性调控，而只有少数斑点对应于所有攻击情况下（包括用脂多糖攻击后）发生改变的蛋白质。所有上调的蛋白质都可归为免疫系统的特定方面，因为它们在无菌针刺幼虫的血淋巴中并未增加。除了已知的免疫蛋白外，还鉴定出了未注释的蛋白质，如CG4306蛋白，在当今所有后生动物基因组数据库中，该蛋白都有功能未知的同源物。
