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通过质粒DNA单次脾内免疫制备抗SARS-CoV刺突蛋白的单克隆抗体。

Production of a monoclonal antibody against SARS-CoV spike protein with single intrasplenic immunization of plasmid DNA.

作者信息

Yu Xiao Fei, Liang Lin Hui, She Ming, Liao Xiao Long, Gu Jie, Li Yan Han, Han Zhong Chao

机构信息

State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College, 288 Nanjing Road, Tianjin 300020, PR China.

出版信息

Immunol Lett. 2005 Sep 15;100(2):177-81. doi: 10.1016/j.imlet.2005.03.015.

Abstract

Severe acute respiratory syndrome (SARS) is a highly infectious disease caused by a novel coronavirus (SARS-CoV). Specific monoclonal antibodies (mAbs) against the SARS-CoV are vital for early diagnosis and pathological studies of SARS. Direct intrasplenic inoculation of plasmid DNA encoding antigen is an effective and fast approach to generate specific mAb when the protein antigen is difficult to prepare or dangerous in use. In this study, we selected one fragment of SARS-CoV spike protein (S1-(3)) as antigenic determinant by immunoinformatics. Single intrasplenic immunization of plasmid DNA encoding S1-(3) induced anti-spike protein antibodies. We established one hybridoma cell line secreting specific mAb and evaluated this mAb with murine leukemia virus pseudotyped with SARS-CoV spike protein (MLV/SARS-CoV). The mAb could recognize the spike protein on the MLV/SARS-CoV-infected Vero E6 cells albeit with no neutralizing effect on the infectivity of the pseudotype virus. Our results show that a single-shot intrasplenic DNA immunization is efficient for the production of specific mAb against SARS spike protein, and a linear epitope of the spike protein is recognized in this study.

摘要

严重急性呼吸综合征(SARS)是一种由新型冠状病毒(SARS-CoV)引起的高传染性疾病。针对SARS-CoV的特异性单克隆抗体(mAb)对于SARS的早期诊断和病理研究至关重要。当蛋白质抗原难以制备或使用危险时,直接脾内接种编码抗原的质粒DNA是产生特异性mAb的有效且快速的方法。在本研究中,我们通过免疫信息学选择了SARS-CoV刺突蛋白的一个片段(S1-(3))作为抗原决定簇。编码S1-(3)的质粒DNA单次脾内免疫诱导产生了抗刺突蛋白抗体。我们建立了一个分泌特异性mAb的杂交瘤细胞系,并用携带SARS-CoV刺突蛋白的鼠白血病病毒假型(MLV/SARS-CoV)对该mAb进行了评估。该mAb能够识别MLV/SARS-CoV感染的Vero E6细胞上的刺突蛋白,尽管对假型病毒的感染性没有中和作用。我们的结果表明,单次脾内DNA免疫对于产生针对SARS刺突蛋白的特异性mAb是有效的,并且在本研究中识别出了刺突蛋白的一个线性表位。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/686d/7112869/68da76b0c701/gr1.jpg

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本文引用的文献

1
SARS-CoV antibody prevalence in all Hong Kong patient contacts.
Emerg Infect Dis. 2004 Sep;10(9):1653-6. doi: 10.3201/eid1009.040155.
2
Immunogenicity of SARS inactivated vaccine in BALB/c mice.
Immunol Lett. 2004 Sep;95(2):139-43. doi: 10.1016/j.imlet.2004.06.014.
4
Retroviral vectors pseudotyped with severe acute respiratory syndrome coronavirus S protein.
J Virol. 2004 Sep;78(17):9007-15. doi: 10.1128/JVI.78.17.9007-9015.2004.
5
Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus.
J Virol Methods. 2004 Sep 1;120(1):87-96. doi: 10.1016/j.jviromet.2004.04.009.
6
Severe acute respiratory syndrome coronavirus spike protein expressed by attenuated vaccinia virus protectively immunizes mice.
Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6641-6. doi: 10.1073/pnas.0401939101. Epub 2004 Apr 19.
7
Induction of SARS-nucleoprotein-specific immune response by use of DNA vaccine.
Immunol Lett. 2004 Apr 15;92(3):237-43. doi: 10.1016/j.imlet.2004.01.001.
8
Phylogenomics and bioinformatics of SARS-CoV.
Trends Microbiol. 2004 Mar;12(3):106-11. doi: 10.1016/j.tim.2004.01.005.
9
Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments.
Biochem Biophys Res Commun. 2004 Mar 19;315(4):1134-9. doi: 10.1016/j.bbrc.2004.01.166.

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