Kirat D, Inoue H, Iwano H, Hirayama K, Yokota H, Taniyama H, Kato S
Department of Veterinary Physiology, School of Veterinary Medicine, Rakuno Gakuen University, 582-1 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
Res Vet Sci. 2005 Aug;79(1):45-50. doi: 10.1016/j.rvsc.2004.11.007. Epub 2005 Jan 20.
In the present study the expression and distribution of monocarboxylate transporter 1 (MCT1) along the gastrointestinal tract (rumen, reticulum, omasum, abomasum, duodenum, jejunum, ileum, cecum and colon) of calves were investigated on both mRNA and protein levels. The expression of MCT1 protein and its distribution were determined by Western blotting and immunohistochemical staining, respectively by using antibody for MCT1. MCT1 protein was visualized as a 43-kDa band on immunoblots of the membrane proteins prepared from the various regions examined, and it was more highly expressed in forestomach and large intestine than in abomasum and small intestine. With the use of reverse transcriptase-polymerase chain reaction, mRNA encoding for MCT1 was demonstrated in the different tissues examined. The immunohistochemical study confirmed the Western blot findings and showed strong MCT1 immunopositive staining in the stratified squamous epithelia of the forestomach as well as the epithelial cells lining the digestive tract in the cecum, proximal colon, and distal colon. The results suggest that MCT1 may play a role in the transport of SCFA and their metabolites in the gastrointestinal tract of bovines.
在本研究中，从mRNA和蛋白质水平对犊牛胃肠道（瘤胃、网胃、瓣胃、皱胃、十二指肠、空肠、回肠、盲肠和结肠）中单羧酸转运蛋白1（MCT1）的表达和分布进行了研究。分别使用抗MCT1抗体，通过蛋白质免疫印迹法和免疫组织化学染色法测定MCT1蛋白的表达及其分布。在从所检测的各个区域制备的膜蛋白免疫印迹上，MCT1蛋白显示为一条43 kDa的条带，其在前胃和大肠中的表达高于皱胃和小肠。通过逆转录聚合酶链反应，在所检测的不同组织中证实了编码MCT1的mRNA的存在。免疫组织化学研究证实了蛋白质免疫印迹的结果，并在前胃的复层鳞状上皮以及盲肠、近端结肠和远端结肠的消化道内衬上皮细胞中显示出强烈的MCT1免疫阳性染色。结果表明，MCT1可能在牛胃肠道中短链脂肪酸及其代谢产物的转运中发挥作用。
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