Kirat D, Inoue H, Iwano H, Hirayama K, Yokota H, Taniyama H, Kato S
Department of Veterinary Physiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
Vet J. 2006 May;171(3):462-7. doi: 10.1016/j.tvjl.2004.12.009.
In this study, we investigated the tissue distribution and expression of monocarboxylate transporter 1 (MCT1) along the gastrointestinal tract of sheep. Western blot analysis suggested the presence of MCT1 as a 43-kDa protein in immunoblots of membranes from the various tissues examined. The results of Western blotting were further confirmed by immunohistochemical studies, which revealed intense immunoreactivity for the MCT1 protein in the forestomach (rumen, reticulum and omasum) and large intestine (caecum, proximal and distal colon). Moderate reactivity, however, was detected in the abomasum, while no immunoreactivity could be seen in any regions of the small intestine examined. Furthermore, MCT1 was expressed at the mRNA level as determined by reverse transcriptase polymerase chain reaction (RT-PCR), which showed a band of the expected size (300 bp) in all tissues examined. From these results we concluded that MCT1 protein is highly expressed and distributed in the stomach and large intestine of sheep suggesting that MCT1 may play a significant role in the transport of short chain fatty acids and their metabolites in the gastrointestinal tract of ruminants.
在本研究中，我们调查了单羧酸转运蛋白1（MCT1）在绵羊胃肠道中的组织分布及表达情况。蛋白质免疫印迹分析表明，在所检测的各种组织的膜免疫印迹中，存在分子量为43 kDa的MCT1蛋白。蛋白质免疫印迹结果通过免疫组织化学研究进一步得到证实，该研究显示，在前胃（瘤胃、网胃和瓣胃）和大肠（盲肠、近端结肠和远端结肠）中，MCT1蛋白呈现强烈的免疫反应性。然而，在皱胃中检测到中等强度的反应性，而在所检测的小肠任何区域均未观察到免疫反应性。此外，通过逆转录聚合酶链反应（RT-PCR）确定，MCT1在mRNA水平表达，在所检测的所有组织中均显示出预期大小（300 bp）的条带。从这些结果我们得出结论，MCT1蛋白在绵羊的胃和大肠中高度表达且分布广泛，这表明MCT1可能在反刍动物胃肠道中短链脂肪酸及其代谢产物的转运中发挥重要作用。
