Shimoyama Yumiko, Kirat Doaa, Akihara Yuko, Kawasako Kazufumi, Komine Misa, Hirayama Kazuko, Matsuda Kazuya, Okamoto Minoru, Iwano Hidetomo, Kato Seiyu, Taniyama Hiroyuki
Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
J Vet Med Sci. 2007 Jun;69(6):599-604. doi: 10.1292/jvms.69.599.
In this study, the expression and distribution of monocarboxyolate transporter 1 (MCT1) along the intestines (duodenum, jejunum, ileum, cecum, colon and rectum) of dogs were investigated at both the mRNA and protein levels. The expression of MCT1 protein and its distribution were confirmed by Western blotting and immunohistochemical staining using the antibody for MCT1. We identified mRNA coding for MCT1 and a 43-kDa band of MCT1 protein in all regions from the duodenum to the rectum. Immunoreactive staining for MCT1 was also observed in epithelial cells throughout the intestines. MCT1 immunoreactivity was greater in the large intestine than in the small intestine. MCT1 protein was predominantly expressed on the basolateral membranes along intestinal epithelial cells, suggesting that MCT1 may play an important role in lactate efflux and transport of short-chain fatty acids (SCFAs) to the bloodstream across the basolateral membranes of the dog intestine.
在本研究中，在mRNA和蛋白质水平上研究了单羧酸转运体1（MCT1）在犬肠道（十二指肠、空肠、回肠、盲肠、结肠和直肠）中的表达和分布。使用针对MCT1的抗体，通过蛋白质印迹法和免疫组织化学染色来确认MCT1蛋白的表达及其分布。我们在从十二指肠到直肠的所有区域中鉴定出编码MCT1的mRNA和一条43 kDa的MCT1蛋白条带。在整个肠道的上皮细胞中也观察到了MCT1的免疫反应性染色。MCT1在大肠中的免疫反应性高于小肠。MCT1蛋白主要沿肠上皮细胞的基底外侧膜表达，这表明MCT1可能在乳酸外流以及短链脂肪酸（SCFAs）通过犬肠道基底外侧膜转运至血液中发挥重要作用。
