谷氨酸能突触后致密蛋白的个体发生
Ontogeny of postsynaptic density proteins at glutamatergic synapses.
作者信息
Petralia Ronald S, Sans Nathalie, Wang Ya-Xian, Wenthold Robert J
机构信息
Laboratory of Neurochemistry, NIDCD/NIH, 50/4142, 50 South Drive MSC 8027, Bethesda, MD 20892-8027, USA.
出版信息
Mol Cell Neurosci. 2005 Jul;29(3):436-52. doi: 10.1016/j.mcn.2005.03.013.
In glutamatergic synapses, glutamate receptors (GluRs) associate with many other proteins involved in scaffolding and signal transduction. The ontogeny of these postsynaptic density (PSD) proteins involves changes in their composition during development, paralleling changes in GluR type and function. In the CA1 region of the hippocampus, at postnatal day 2 (P2), many synapses already have a distinct PSD. We used immunoblot analysis, subcellular fractionation, and quantitative immunogold electron microscopy to examine the distribution of PSD proteins during development of the hippocampus. Synapses at P2 contained substantial levels of NR1 and NR2B and most GluR-associated proteins, including SAP102, SynGAP, the chain of proteins from GluRs/SAP102 through GKAP/Shank/Homer and metabotropic glutamate receptors, and the adhesion factors, cadherin, catenin, neuroligin, and Nr-CAM. Development was marked by substantial decreases in NR2B and SAP102 and increases in NR2A, PSD-95, AMPA receptors, and CaMKII. Other components showed more moderate changes.
在谷氨酸能突触中，谷氨酸受体（GluRs）与许多其他参与支架构建和信号转导的蛋白质相关联。这些突触后致密物（PSD）蛋白的个体发生涉及发育过程中其组成的变化，这与GluR类型和功能的变化平行。在海马体的CA1区域，出生后第2天（P2）时，许多突触已经有明显的PSD。我们使用免疫印迹分析、亚细胞分级分离和定量免疫金电子显微镜来检查海马体发育过程中PSD蛋白的分布。P2时的突触含有大量的NR1和NR2B以及大多数与GluR相关的蛋白，包括SAP102、SynGAP、从GluRs/SAP102到GKAP/Shank/Homer和代谢型谷氨酸受体的蛋白质链，以及黏附因子、钙黏蛋白、连环蛋白、神经连接蛋白和Nr-CAM。发育过程的特点是NR2B和SAP102大幅减少，而NR2A、PSD-95、AMPA受体和CaMKII增加。其他成分显示出更适度的变化。
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