Zou Wang-yuan, Guo Qu-lian, Wang E, Cai Jin
Department of Anesthesiology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2005 Apr;30(2):157-61.
To evaluate the immunological function in rats with formalin inflammatory pain through intrathecal pumping different dosages of morphine.
Thirty-two Sprague-Dawley rats were randomly divided into 4 groups (n = 8 in each group): saline group (NS) and morphine group included M1 group (10 microg/h) , M2 group (5 microg/h), and M3 group (2.5 microg/h). Chronic intrathecal catheterization was performed under anesthesia with 10% chloral hydrate (300-350) mg/kg according to M2 group (5 microg/h) and M3 group (2. 5 microg/h). Chronic intrathecal catheterization was modified Yaksh's. After 7 days, pain intensity scoring (PIS) was utilized to assess antinociceptive effect of morphine. And spleens were aseptically removed to obtain splenic cells. T lymphocyte function was evaluated based on Concanavalin-A induced splenocyte proliferation. A modified lactic acid dehydrogenase release assay was used to assess NK cell activity. Phenotypic expression of cell surface markers of T lymphocyte subsets (CD3+, CD3+ CD4+, CD3+ CD8+, and CD4+ / CD8+ ) and NK cell ( CD161+) in the spleen were analyzed by flow cytometry.
Compared with the NS group, PIS of morphine group decreased obviously (P < 0.01) and was dose-dependent in the early and late phase of formalin pain, but there were no significant differences among morphine groups. Spleen index, splenocyte proliferation and NK cell activity were significantly suppressed by intrathecal pumping morphine. Phenotypic expression of T lymphocyte subsets and NK cell assessed by flow cytometry were different from the control group in all morphine groups.
There was significant antinociception of intrathecal pumping morphine. After intrathecal pumping different dosages of morphine (10 microg/h,5 microg/h, and 2.5 microg/h), the function of cellular immunity was suppressed and was dose-dependent.
通过鞘内注射不同剂量吗啡评估福尔马林炎性疼痛大鼠的免疫功能。
将32只Sprague-Dawley大鼠随机分为4组（每组n = 8）：生理盐水组（NS）和吗啡组，吗啡组包括M1组（10微克/小时）、M2组（5微克/小时）和M3组（2.5微克/小时）。按照M2组（5微克/小时）和M3组（2.5微克/小时）的方法，在10%水合氯醛（300 - 350）毫克/千克麻醉下进行慢性鞘内导管插入术。慢性鞘内导管插入术采用改良的Yaksh法。7天后，采用疼痛强度评分（PIS）评估吗啡的镇痛效果。无菌取出脾脏获得脾细胞。基于刀豆蛋白A诱导的脾细胞增殖评估T淋巴细胞功能。采用改良的乳酸脱氢酶释放试验评估NK细胞活性。通过流式细胞术分析脾脏中T淋巴细胞亚群（CD3 +、CD3 + CD4 +、CD3 + CD8 +和CD4 + / CD8 +）和NK细胞（CD161 +）的细胞表面标志物的表型表达。
与NS组相比，吗啡组的PIS明显降低（P < 0.01），在福尔马林疼痛的早期和晚期呈剂量依赖性，但吗啡组之间无显著差异。鞘内注射吗啡显著抑制脾脏指数、脾细胞增殖和NK细胞活性。流式细胞术评估的所有吗啡组中T淋巴细胞亚群和NK细胞的表型表达与对照组不同。
鞘内注射吗啡有显著的镇痛作用。鞘内注射不同剂量吗啡（分别为10微克/小时、5微克/小时和2.5微克/小时）后，细胞免疫功能受到抑制且呈剂量依赖性。
