Colgan S P, Blancquaert A M, Thrall M A, Bruyninckx W J
Department of Microbiology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
Vet Immunol Immunopathol. 1992 Mar;31(3-4):205-27. doi: 10.1016/0165-2427(92)90010-n.
The in vitro migratory responses of neutrophils of homozygote and heterozygote Chediak-Higashi cats were defective in an under-agarose assay when compared to the behavior of phagocytes of control cats. The linear distances traversed by the leading front of migrating Chediak-Higashi neutrophils toward streptococcal culture supernatant, zymosan-activated serum or buffer were reduced and smaller numbers of Chediak-Higashi phagocytes populated the resulting migration areas than did cells of control animals. The relative migration parameters of the Chediak-Higashi phagocytes, however, did not differ from the corresponding parameters of control neutrophils in the presence of streptococcal culture supernatant. Therefore, phagocytes of homozygote and heterozygote Chediak-Higashi cats recognized and responded equally well to the bacterial stimuli as did cells of control animals but traveled shorter distances primarily because of a reduced inherent motility. Similar results were also obtained when the feline phagocytes were attracted by zymosan-activated serum. In addition the relative migration parameters of the neutrophils of homozygote Chediak-Higashi cats were reduced and the normalized spatial distributions of their migrating cells were significantly different in the presence of 100% and 20% zymosan-activated serum when compared to the corresponding migration parameters of carrier and control animals. Defective recognition or responses to the higher concentrations of these host-derived attractants complicated, therefore, the already reduced inherent motility of the phagocytes of homozygote Chediak-Higashi cats.
与对照猫的吞噬细胞行为相比，纯合子和杂合子切迪阿克-东综合征（Chediak-Higashi）猫的中性粒细胞在琼脂糖下测定中的体外迁移反应存在缺陷。与对照动物的细胞相比，迁移的切迪阿克-东综合征中性粒细胞前沿向链球菌培养上清液、酵母聚糖激活血清或缓冲液移动的直线距离缩短，且进入迁移区域的切迪阿克-东综合征吞噬细胞数量较少。然而，在存在链球菌培养上清液的情况下，切迪阿克-东综合征吞噬细胞的相对迁移参数与对照中性粒细胞的相应参数并无差异。因此，纯合子和杂合子切迪阿克-东综合征猫的吞噬细胞与对照动物的细胞一样，对细菌刺激的识别和反应良好，但移动距离较短，主要是因为固有运动性降低。当猫的吞噬细胞被酵母聚糖激活血清吸引时，也获得了类似的结果。此外，与携带者和对照动物的相应迁移参数相比，在存在100%和20%酵母聚糖激活血清的情况下，纯合子切迪阿克-东综合征猫中性粒细胞的相对迁移参数降低，其迁移细胞的标准化空间分布也存在显著差异。因此，对这些宿主来源的趋化因子较高浓度的识别或反应缺陷，使纯合子切迪阿克-东综合征猫吞噬细胞本已降低的固有运动性变得更加复杂。
