Blancquaert A B, Colgan S P, Bruyninckx W J
Department of Microbiology, College of Veterinary Medicine, Colorado State University, Fort Collins 80523.
Vet Immunol Immunopathol. 1989 Nov 30;23(1-2):85-101. doi: 10.1016/0165-2427(89)90112-8.
To identify the influence of technical factors on the in vitro motility of equine neutrophils towards streptococcus culture supernatant in an under-agarose assay, we studied the changes in eight cell migration parameters. The distances the phagocytes travelled by directed, random and spontaneous migration increased with incubation time, cell concentration and the gelatin and serum contents of the migration plates. The contribution of chemotaxis to the phagocyte migrations, however, decreased simultaneously. The directed and random, though not the spontaneous, migrations of the phagocytes increased also when the chemoattractant wells were placed closer to the cell wells but so did the influence of the chemokinetic activity of the bacterial culture supernatant on phagocyte motility. In contrast, preincubation of migration plates with the chemoattractant, the agarose content of the migration plates and contamination of the granulocytes with non-migrating, mononuclear cells did not substantially affect the in vitro migrations of the neutrophils. The changes in the in vitro motility of the equine neutrophils by these technical factors were, in general, comparable to those reported for human cells attracted by a variety of host-and bacteria-derived chemoattractants.
为了在琼脂糖下测定中确定技术因素对马中性粒细胞向链球菌培养上清液体外运动性的影响，我们研究了八个细胞迁移参数的变化。吞噬细胞通过定向、随机和自发迁移所行进的距离随着孵育时间、细胞浓度以及迁移平板中明胶和血清含量的增加而增加。然而，趋化作用对吞噬细胞迁移的贡献同时降低。当趋化剂孔放置得更靠近细胞孔时，吞噬细胞的定向和随机迁移（但不是自发迁移）也会增加，细菌培养上清液的化学动力学活性对吞噬细胞运动性的影响也会增加。相比之下，用趋化剂预孵育迁移平板、迁移平板的琼脂糖含量以及非迁移单核细胞对粒细胞的污染，对中性粒细胞的体外迁移没有实质性影响。这些技术因素引起的马中性粒细胞体外运动性的变化，总体上与报道的被多种宿主和细菌来源的趋化剂吸引的人类细胞的变化相当。
