Gallin J I, Klimerman J A, Padgett G A, Wolff S M
Blood. 1975 Jun;45(6):863-70.
Chemotaxis of mononuclear leukocytes from humans, mink, and cattle was evaluated in vitro using a morphologic Boyden chamber technique and a new 51-Cr-labeled mononuclear radioassay with a double micropore filter system. Significantly decreased mononuclear leukocyte chemotactic response were noted when human, mink, or cattle Chediak-Higashi cells were tested using autologous serum or endotoxin-activated autolotous serum. A similar Chediak-Higashi mononuclear leukocyte defect was noted in humans when kallikrein or dialyzable transfer factor were used as the chemotactic stimulus. Studies using smaller pore filters in the chemotactic chamber exaggerated the chemotactic defect. Serum from Chediak-Higashi subjects had normal chemotactic activity. Additional studies on the spontaneous (random) locomotion of Chediak-Higashi mononuclear leukocytes revealed normal results when a capillary tube assay system was used, but abnormal results were obtained when a Boyden chamber micropore filter assay was used, demonstrating fundamental differences in these two assays of random locomotion. It is clear from these studies that defective mononuclear leukocyte chemotaxis is another feature of the imparied host defenses in the Chediak-Higashi syndrome that may contribute to the marked susceptibility to pyogenic infections so characteristic of this dease.
采用形态学的博伊登小室技术和一种带有双微孔滤器系统的新型51铬标记单核细胞放射测定法，在体外对来自人类、水貂和牛的单核白细胞的趋化性进行了评估。当使用自体血清或内毒素激活的自体血清对人类、水貂或牛的切迪阿克-东综合征细胞进行测试时，发现单核白细胞趋化反应显著降低。当使用激肽释放酶或可透析转移因子作为趋化刺激物时，在人类中也发现了类似的切迪阿克-东综合征单核白细胞缺陷。在趋化小室中使用孔径更小的滤器进行的研究夸大了趋化缺陷。切迪阿克-东综合征患者的血清具有正常的趋化活性。对切迪阿克-东综合征单核白细胞自发（随机）运动的进一步研究表明，当使用毛细管测定系统时结果正常，但当使用博伊登小室微孔滤器测定时结果异常，这表明这两种随机运动测定方法存在根本差异。从这些研究中可以清楚地看出，单核白细胞趋化缺陷是切迪阿克-东综合征中宿主防御受损的另一个特征，这可能导致该疾病患者对化脓性感染具有明显的易感性。
