Zhang Bing, Jin Wu, Zeng Yanwu, Su Zhixi, Hu Songnian, Yu Jun
James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou Genomics Institute, Hangzhou 310008, China.
Genomics Proteomics Bioinformatics. 2004 Nov;2(4):237-44. doi: 10.1016/s1672-0229(04)02030-3.
Since pig is an important livestock species worldwide, its gene expression has been investigated intensively, but rarely in brain. In order to study gene expression profiles in the pig central nervous system, we sequenced and analyzed 43,122 high-quality 5' end expressed sequence tags (ESTs) from porcine cerebellum, cortex cerebrum, and brain stem cDNA libraries, involving several different prenatal and postnatal developmental stages. The initial ESTs were assembled into 16,101 clusters and compared to protein and nucleic acid databases in GenBank. Of these sequences, 30.6% clusters matched protein databases and represented function known sequences; 75.1% had significant hits to nucleic acid databases and partial represented known function; 73.3% matched known porcine ESTs; and 21.5% had no matches to any known sequences in GenBank. We used the categories defined by the Gene Ontology to survey gene expression in the porcine brain.
由于猪是全球重要的家畜物种，其基因表达已得到深入研究，但在大脑中的研究却很少。为了研究猪中枢神经系统中的基因表达谱，我们对来自猪小脑、大脑皮层和脑干cDNA文库的43122个高质量5'端表达序列标签（EST）进行了测序和分析，涉及几个不同的产前和产后发育阶段。最初的EST被组装成16101个簇，并与GenBank中的蛋白质和核酸数据库进行比较。在这些序列中，30.6%的簇与蛋白质数据库匹配并代表已知功能序列；75.1%与核酸数据库有显著匹配且部分代表已知功能；73.3%与已知猪EST匹配；21.5%与GenBank中任何已知序列均无匹配。我们使用基因本体论定义的类别来研究猪脑中的基因表达。
