Su Zhixi, Dong Xinjiao, Zhang Bing, Zeng Yanwu, Fu Yan, Yu Jun, Hu Songnian
James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou 310008, China.
Sci China C Life Sci. 2006 Feb;49(1):26-36. doi: 10.1007/s11427-005-0181-0.
A total of 28941 ESTs were sequenced from five 5'-directed non-normalized cDNA libraries, which were assembled into 2212 contigs and 5642 singlets using CAP3. These sequences were annotated and clustered into 6857 unique genes, 2072 of which having no functional annotations were considered as novel genes. These genes were further classified into Gene Ontology categories. By comparing the expression profiles, we identified some breed- and developmental-stage-specific gene groups. These genes may be relative to reproductive performance or play important roles in milk synthesis, secretion and mammary involution. The unknown EST sequences and expression profiles at different developmental stages and breeds are very important resources for further research.
从五个5'端定向的非标准化cDNA文库中总共测序了28941条EST，使用CAP3将其组装成2212个重叠群和5642个单拷贝序列。对这些序列进行注释并聚类为6857个独特基因，其中2072个没有功能注释的基因被视为新基因。这些基因进一步被分类到基因本体论类别中。通过比较表达谱，我们鉴定出了一些品种和发育阶段特异性的基因群。这些基因可能与繁殖性能相关，或者在乳汁合成、分泌和乳腺退化中发挥重要作用。不同发育阶段和品种的未知EST序列和表达谱是进一步研究的非常重要的资源。
