Manukhov Ilya V, Mamaeva Daria V, Rastorguev Sergei M, Faleev Nicolai G, Morozova Elena A, Demidkina Tatyana V, Zavilgelsky Gennadii B
State Research Institute of Genetic and Selection of Industrial Microorganisms, 1st Dorozhnii pr. 1, Moscow 117545, Russia.
J Bacteriol. 2005 Jun;187(11):3889-93. doi: 10.1128/JB.187.11.3889-3893.2005.
Citrobacter freundii cells produce L-methionine gamma-lyase when grown on a medium containing L-methionine. The nucleotide sequence of the hybrid plasmid with a C. freundii EcoRI insert of about 3.0 kbp contained two open reading frames, consisting of 1,194 nucleotides and 1,296 nucleotides, respectively. The first one (denoted megL) encoded L-methionine gamma-lyase. The enzyme was overexpressed in Escherichia coli and purified. The second frame encoded a protein belonging to the family of permeases. Regions of high sequence identity with the 3'-terminal part of the C. freundii megL gene located in the same regions of Salmonella enterica serovar Typhimurium, Shigella flexneri, E. coli, and Citrobacter rodentium genomes were found.
弗氏柠檬酸杆菌细胞在含有L-甲硫氨酸的培养基上生长时会产生L-甲硫氨酸γ-裂解酶。带有约3.0 kbp弗氏柠檬酸杆菌EcoRI插入片段的杂交质粒的核苷酸序列包含两个开放阅读框，分别由1194个核苷酸和1296个核苷酸组成。第一个（称为megL）编码L-甲硫氨酸γ-裂解酶。该酶在大肠杆菌中过表达并纯化。第二个阅读框编码一种属于通透酶家族的蛋白质。在鼠伤寒沙门氏菌、福氏志贺氏菌、大肠杆菌和啮齿柠檬酸杆菌基因组的相同区域中发现了与弗氏柠檬酸杆菌megL基因3'末端部分具有高度序列同一性的区域。
