从噬菌体展示cDNA文库中分离肥胖带绦虫抗原及其用于神经囊尾蚴病诊断的评估。

Isolation of the Taenia crassiceps antigens from a phage display cDNA library and evaluation of their use for diagnosis of neurocysticercosis.

作者信息

Robles Yetzi, González Erik, Govezensky Tzipe, Mungia Maria Elena, Acero Gonzalo, Bobes Raul J, Gevorkian Goar, Manoutcharian Karen

机构信息

Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, México DF CP04510, México.

出版信息

Clin Immunol. 2005 Sep;116(3):265-70. doi: 10.1016/j.clim.2005.04.009.

DOI:10.1016/j.clim.2005.04.009

PMID:15905129

Abstract

A novel cDNA cloning strategy consisting in elimination of non-coding DNA sequences from 3' regions of cDNAs was applied to construct the Taenia crassiceps phage displayed cDNA expression library. After biopanning using immune sera, three phage clones expressing T. crassiceps-derived antigens specifically recognizing antibodies present in cerebrospinal fluid and plasma samples from neuroimaging-confirmed neurocysticercosis patients were selected. This novel cloning strategy may be applied to other pathogens allowing rapid identification of peptides/proteins for immunodiagnostic tests.

摘要

一种新的cDNA克隆策略，即从cDNA的3'区域消除非编码DNA序列，被应用于构建猪带绦虫噬菌体展示cDNA表达文库。在使用免疫血清进行生物淘选后，选择了三个表达猪带绦虫衍生抗原的噬菌体克隆，这些抗原能特异性识别神经影像学确诊的神经囊尾蚴病患者脑脊液和血浆样本中存在的抗体。这种新的克隆策略可应用于其他病原体，从而快速鉴定用于免疫诊断测试的肽/蛋白质。