da Silva Ribeiro Vanessa, Manhani Marianna Nascimento, Cardoso Rone, Vieira Carlos Ueira, Goulart Luiz Ricardo, Costa-Cruz Julia Maria
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Avenida Pará, 1720, CEP 38400-902, Uberlândia, Minas Gerais, Brazil.
Immunol Lett. 2010 Apr 8;129(2):94-9. doi: 10.1016/j.imlet.2010.01.008. Epub 2010 Feb 4.
Neurocysticercosis (NC), caused by Taenia solium, is the most common infection caused by helminthes of the human central nervous system. In this study, a random peptide phage display library was used to isolate peptide ligands as potential markers for neurocysticercosis diagnosis, because occurrence of cross-reactions with other helminthes species in the current used markers. We selected different peptides using IgG purified from pooled sera of neurocysticercosis patients. To investigate the diagnostic potential of recombinant peptides, we have tested different panels of serum samples by Phage-ELISA, and 10 phage clones strongly bound to the anti-T. solium IgGs in NC sera, with an accuracy range from 84.2% to 95%. The phage clones, NC(4)1 and NC(2)8, presented the highest sensitivity and specificity (100%), respectively, and most important, some phage clones did not react with patients' sera from Echinococcus granulosus infected patients. The validation with a competitive ELISA assay demonstrated that the selected phages could mimic T. solium epitopes and bind specifically to the pool of NC sera. Finally, the two recombinant antigens may become potential biomarkers for serodiagnosis of NC, and the Phage-ELISA demonstrated to be a very good assay, being reproducible, simple, fast, and low-cost due to its production through Escherichia coli culture, allowing a high throughput screening of NC.
神经囊尾蚴病（NC）由猪带绦虫引起，是人类中枢神经系统最常见的蠕虫感染。在本研究中，使用随机肽噬菌体展示文库分离肽配体作为神经囊尾蚴病诊断的潜在标志物，因为目前使用的标志物会与其他蠕虫物种发生交叉反应。我们使用从神经囊尾蚴病患者混合血清中纯化的IgG选择了不同的肽。为了研究重组肽的诊断潜力，我们通过噬菌体酶联免疫吸附测定（Phage-ELISA）检测了不同组别的血清样本，10个噬菌体克隆与神经囊尾蚴病血清中的抗猪带绦虫IgG强烈结合，准确率在84.2%至95%之间。噬菌体克隆NC(4)1和NC(2)8分别表现出最高的敏感性和特异性（100%），最重要的是，一些噬菌体克隆不与细粒棘球绦虫感染患者的血清发生反应。竞争性酶联免疫吸附测定验证表明，所选噬菌体可以模拟猪带绦虫表位并特异性结合神经囊尾蚴病血清池。最后，这两种重组抗原可能成为神经囊尾蚴病血清诊断的潜在生物标志物，并且噬菌体酶联免疫吸附测定被证明是一种非常好的检测方法，由于其通过大肠杆菌培养产生，具有可重复性、简单、快速和低成本的特点，允许对神经囊尾蚴病进行高通量筛选。
