González Erik, Robles Yetzi, Govezensky Tzipe, Bobes Raul J, Gevorkian Goar, Manoutcharian Karen
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México DF, México.
J Biomol Screen. 2010 Dec;15(10):1268-73. doi: 10.1177/1087057110385229. Epub 2010 Oct 25.
In this study, the authors have generated a tapeworm Taenia solium genomic DNA expression library where foreign peptides/proteins were fused to N-termini of M13 cpVIII and expressed at a high copy number on the phage surface, and they showed that this library may be used in bioselection against antipathogen immune sera, allowing the identification of disease-related antigens recognizing antibodies present in clinical samples. They isolated 2 phage clones expressing T. solium-derived antigens specifically reacting with antibodies present in plasma and cerebrospinal fluid samples of neuroimaging-confirmed neurocysticercosis patients. The described antigen discovery strategy may be used for the direct identification of antigens useful for host-pathogen interaction studies as well as for the development of molecular vaccines and diagnostics.
在本研究中，作者构建了一个猪带绦虫基因组DNA表达文库，其中外源肽/蛋白质与M13 cpVIII的N端融合，并以高拷贝数在噬菌体表面表达。他们表明，该文库可用于针对抗病原体免疫血清的生物筛选，从而鉴定临床样本中存在的可识别疾病相关抗原的抗体。他们分离出了2个噬菌体克隆，这些克隆表达的猪带绦虫衍生抗原能与神经影像学确诊的神经囊尾蚴病患者血浆和脑脊液样本中的抗体特异性反应。所描述的抗原发现策略可用于直接鉴定对宿主-病原体相互作用研究有用的抗原，以及用于开发分子疫苗和诊断方法。
