Onwuliri C O, Nwosu N U, Onwuliri V A
Department of Zoology, University of Jos, Nigeria.
Angew Parasitol. 1992 Feb;33(1):51-5.
The concentration of carbohydrates, proteins, and lipids in the whole worm, gut, and body musculature of Fasciola gigantica was investigated from worms collected from cattle slaughtered in the Jos abattoir. Protein accounted for the highest concentration of the constituents constituting 62.59 +/- 0.86% of the dry weight of the fluke. This was followed by lipid with 28.15 +/- 0.86% of the dry weight while carbohydrate (glycogen) was 6.29 +/- 0.11% of the dry weight. Thin-layer chromatography revealed the presence of six distinct bands of neutral lipids, namely: monoglycerides, 1,2-diglycerides, 1,3-diglycerides, free fatty acids, triglycerides and sterol esters from both whole worm homogenate and fractions of the gut and muscle tissues. In the case of phospholipids, lysophosphatidyl choline, sphingomyeline, phosphatidyl choline and phosphatidyl-ethanol amine were recovered. When the free fatty acids were subjected to further fractionation, palmitic, stearic, as well as oleic acids were recovered in appreciable quantities indicating that lipids are being catabolized in these trematodes.
从乔斯屠宰场宰杀的牛体内采集肝片吸虫，对其整个虫体、肠道和体肌中的碳水化合物、蛋白质和脂质浓度进行了研究。蛋白质在构成吸虫干重的成分中浓度最高，占62.59±0.86%。其次是脂质，占干重的28.15±0.86%，而碳水化合物（糖原）占干重的6.29±0.11%。薄层色谱显示，从整个虫体匀浆以及肠道和肌肉组织部分中均存在六种不同的中性脂质条带，即：甘油单酯、1,2 - 甘油二酯、1,3 - 甘油二酯、游离脂肪酸、甘油三酯和甾醇酯。在磷脂方面，回收了溶血磷脂酰胆碱、鞘磷脂、磷脂酰胆碱和磷脂酰乙醇胺。当对游离脂肪酸进行进一步分离时，回收了大量的棕榈酸、硬脂酸以及油酸，这表明这些吸虫中的脂质正在被分解代谢。
