Sauerwald Anselm, Sitaramaiah Devarasetty, McCloskey James A, Söll Dieter, Crain Pamela F
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA.
FEBS Lett. 2005 May 23;579(13):2807-10. doi: 10.1016/j.febslet.2005.04.014. Epub 2005 Apr 21.
Post-transcriptionally modified nucleosides are constituents of transfer RNA (tRNA) that are known to influence tertiary structure, stability and coding properties. Modifications in unfractionated tRNA from the phylogenetically unique archaeal methanogen Methanopyrus kandleri (optimal growth temperature 98 degrees C) were studied using liquid chromatography-mass spectrometry to establish the extent to which they might differ from those of other methanogens. The exceptionally diverse population of nucleosides included four new nucleosides of unknown structure, and one that was characterized as N(6)-acetyladenosine, a new RNA constituent. The nucleoside modification pattern in M. kandleri tRNA is notably different from that of other archaeal methanogens, and is closer to that of the thermophilic crenarchaeota.
转录后修饰的核苷是转运RNA（tRNA）的组成部分，已知其会影响三级结构、稳定性和编码特性。利用液相色谱-质谱法研究了系统发育独特的古生菌甲烷嗜热菌（最佳生长温度98摄氏度）未分级tRNA中的修饰，以确定其与其他产甲烷菌的差异程度。异常多样的核苷群体包括四种结构未知的新核苷，以及一种被鉴定为N（6）-乙酰腺苷的新RNA成分。甲烷嗜热菌tRNA中的核苷修饰模式与其他古生菌产甲烷菌明显不同，更接近嗜热泉古菌。
