McCloskey J A, Liu X H, Crain P F, Bruenger E, Guymon R, Hashizume T, Stetter K O
Department of Medicinal Chemistry, University of Utah, Salt Lake City, UT 84112, USA.
Nucleic Acids Symp Ser. 2000(44):267-8. doi: 10.1093/nass/44.1.267.
In the RNA of hyperthermophiles, which grow optimally between 80 degrees C and 106 degrees C, posttranscriptional modification has been identified as a leading mechanism of structural stabilization. Particularly in the Archaeal evolutionary domain these modifications are expressed as a structurally diverse array of modification motifs, many of which include ribose methylation. Using mass spectrometric techniques we have examined the posttranscriptional modifications in unfractionated tRNA from the remarkable organism Pyrolobus fumarii, which grows optimally at 106 degrees C, but up to 113 degrees C (Blöchl et al. (1997), Extremophiles, 1, 14-21). Twenty-six modified nucleosides were detected, 11 of which are methylated in ribose. A new RNA nucleoside, 1,2'-O-dimethylguanosine (m1Gm) was characterized and the structure confirmed by chemical synthesis.
在最适生长温度介于80摄氏度至106摄氏度之间的嗜热菌的RNA中，转录后修饰已被确定为结构稳定的主要机制。特别是在古细菌进化域中，这些修饰表现为一系列结构多样的修饰基序，其中许多包括核糖甲基化。我们使用质谱技术研究了来自非凡生物嗜热栖热袍菌（Pyrolobus fumarii）的未分级tRNA中的转录后修饰，该生物最适生长温度为106摄氏度，但最高可达113摄氏度（布洛赫尔等人（1997年），《极端微生物》，第1卷，第14 - 21页）。检测到26种修饰核苷，其中11种在核糖中发生甲基化。一种新的RNA核苷，1,2'-O-二甲基鸟苷（m1Gm）得到了表征，并通过化学合成确认了其结构。
