Greenlee Anne R, Ellis Tammy M, Berg Richard L, Mercieca Michael D
Marshfield Clinic Research Foundation, Marshfield, WI, USA.
Reprod Toxicol. 2005 Jul-Aug;20(2):229-38. doi: 10.1016/j.reprotox.2005.02.004.
Pregnancy outcomes were evaluated following uterine transfer of murine preimplantation embryos exposed in vitro to the estrogenic pesticide o,p'-dichlorodiphenyltrichloroethane (o,p'-DDT). Single-cell embryos were incubated 72 h in medium droplets containing 0.1% ethanol (control) or 0.1 microg/ml o,p'-DDT (pesticide). Morula and preblastocyst embryos were transferred in groups of eight to right uterine horns of pseudopregnant mice (n=111) and pups (n=132) were evaluated at Caesarean-section (C-section). In vitro exposure to o,p'-DDT reduced development to morula (P<0.001) and modestly increased blastomere apoptosis (P=0.05). However, treatment differences were not detected for implantation rates (35% versus 39%; P=0.64), pup numbers per dam (2.3 versus 1.9; P=0.36), transfer efficiencies (16% versus 14%; P=0.53), fetal weights (1.56 g versus 1.57 g; P=0.91), skeletal abnormalities (55% versus 66%; P=0.47), or male ratios (54.8% versus 53.8%; P=1.0). In vitro exposure of preimplantation embryos to 0.1 microg/ml o,p'-DDT for 72 h resulted in no measurable effects on subsequent implantation or pup characteristics at C-section.
在体外将小鼠植入前胚胎暴露于雌激素类农药邻，对'-二氯二苯三氯乙烷（o，p'-滴滴涕）后，评估其妊娠结局。单细胞胚胎在含有0.1%乙醇（对照）或0.1微克/毫升o，p'-滴滴涕（农药）的培养基液滴中孵育72小时。桑椹胚和早期囊胚胚胎以每组8个的数量转移到假孕小鼠的右子宫角（n = 111），并在剖腹产时评估幼崽（n = 132）。体外暴露于o，p'-滴滴涕会降低桑椹胚的发育率（P<0.001），并适度增加卵裂球凋亡（P = 0.05）。然而，在着床率（35%对39%；P = 0.64）、每只母鼠的幼崽数量（2.3对1.9；P = 0.36）、移植效率（16%对14%；P = 0.53）、胎儿体重（1.56克对1.57克；P = 0.91）、骨骼异常（55%对66%；P = 0.47）或雄性比例（54.8%对53.8%；P = 1.0）方面未检测到处理差异。体外将植入前胚胎暴露于0.1微克/毫升o，p'-滴滴涕72小时对随后的着床或剖腹产时的幼崽特征没有可测量的影响。
