Kapulkin Wadim J, Hiester Brian G, Link Christopher D
Institute for Behavioral Genetics, Campus Box 447, University of Colorado, Boulder, CO 80309, USA.
FEBS Lett. 2005 Jun 6;579(14):3063-8. doi: 10.1016/j.febslet.2005.04.062.
We have identified a particularly clear case of compensatory transcriptional regulation among ER chaperones in Caenorhabditis elegans using a GFP reporter transgene that is under the control of the promoter of hsp-4, a C. elegans homolog of GRP-78/BiP. Knockdown by RNA interference of 9 known or predicted ER chaperones induced hsp-4 upregulation via the ire-1/xbp-1 signaling cascade employed in the unfolded protein response. We show that this compensatory regulation is specific for ER chaperones, not dependent on RNA interference, and required for maintaining viability in worms containing a deletion of the hsp-3 gene.
我们利用一个受hsp-4启动子控制的绿色荧光蛋白（GFP）报告转基因，在秀丽隐杆线虫中确定了内质网（ER）伴侣蛋白间补偿性转录调控的一个特别明显的例子。hsp-4是GRP-78/BiP的秀丽隐杆线虫同源物。通过RNA干扰敲低9种已知或预测的内质网伴侣蛋白，可通过未折叠蛋白反应中使用的ire-1/xbp-1信号级联诱导hsp-4上调。我们表明，这种补偿性调控对内质网伴侣蛋白具有特异性，不依赖于RNA干扰，并且对于维持hsp-3基因缺失的线虫的生存能力是必需的。
