Jacobi U, Kaiser M, Richter H, Audring H, Sterry W, Lademann J
Department of Dermatology, Medical Faculty Charité, Berlin, Germany.
Skin Pharmacol Physiol. 2005 Jul-Aug;18(4):175-9. doi: 10.1159/000085862. Epub 2005 May 20.
The removal of the stratum corneum (SC) using adhesive tapes is a common technique in cutaneous studies. The determination of the varying amounts of the SC removed would be a helpful tool in such investigations. In the present study, the cell layers of porcine SC were counted before and after removal of several tape strips using histological techniques. In addition, the pseudo-absorption of the corneocytes reflecting the amount of these cells was determined using spectroscopy. Different amounts of SC were removed using 20 tape strips. The spectroscopically determined data correlate linearly with the number of removed cell layers. Based on these results, the pseudo-absorption of the corneocytes can be used to calculate the absolute number of cell layers removed with a standard deviation of less than 11%. In this way, the SC can be quantified using the procedure of tape stripping in combination with the spectroscopic determination of the corneocytes.
使用胶带去除角质层（SC）是皮肤研究中的一种常用技术。确定去除的不同量的SC将是此类研究中的一个有用工具。在本研究中，使用组织学技术对去除几条胶带前后猪SC的细胞层数进行了计数。此外，使用光谱法测定了反映这些细胞数量的角质形成细胞的假吸收。使用20条胶带去除了不同量的SC。光谱测定的数据与去除的细胞层数呈线性相关。基于这些结果，角质形成细胞的假吸收可用于计算去除的细胞层的绝对数量，标准差小于11%。通过这种方式，可以结合胶带剥离程序和角质形成细胞的光谱测定来量化SC。
