Lee Sang Kil, Kim Hyo Jong, Chi Sung Gil, Jang Jae Young, Nam Ki Deok, Kim Nam Hoon, Joo Kwang Ro, Dong Seok Ho, Kim Byung Ho, Chang Young Woon, Lee Joung Il, Chang Rin
Department of Internal Medicine, Kyung Hee University College of Medicine, Seoul, Korea.
Korean J Gastroenterol. 2005 May;45(5):328-34.
BACKGROUND/AIMS: Saccharomyces boulardii (S. boulardii) has been reported to be beneficial in the treatment of inflammatory bowel disease, however, little is known about its mechanism of action. Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) is recently found to regulate inflammation in intestinal epithelial cells. We hypothesized that the anti-inflammatory effects of S. boulardii are mediated, in part, through PPAR-gamma. To test this hypothesis, we examined the ability of S. boulardii to modulate the expression of PPAR-gamma in human colon cells.
Effects of S. boulardii on survival and proliferation of HT-29 human colon cells were assessed by MTT and [3H]thymidine incorporation assays. PPAR-gamma expression was assessed by Western blot and RT-PCR. Induction of interleukin-8 (IL-8) expression by tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), or lipopolysaccharide (LPS) was assessed by RT-PCR.
S. boulardii did not affect viability and proliferation of HT-29 cells. S. boulardii up-regulated PPAR-gamma expression at both mRNA and protein levels. Pretreatment of HT-29 cells with S. boulardii blocked PPAR-gamma down-regulation by TNF-alpha, IL-1beta, or LPS, whereas it ameliorated IL-8 response to these proinflammatory factors.
S. boulardii stimulates PPAR-gamma expression and reduces response of human colon cells to proinflammatory cytokines.
背景/目的：据报道，布拉酵母菌（S. boulardii）对炎症性肠病的治疗有益，然而，其作用机制尚不清楚。最近发现过氧化物酶体增殖物激活受体γ（PPAR-γ）可调节肠道上皮细胞中的炎症。我们推测布拉酵母菌的抗炎作用部分是通过PPAR-γ介导的。为了验证这一假设，我们研究了布拉酵母菌调节人结肠细胞中PPAR-γ表达的能力。
通过MTT和[3H]胸苷掺入试验评估布拉酵母菌对HT-29人结肠细胞存活和增殖的影响。通过蛋白质印迹法和逆转录-聚合酶链反应（RT-PCR）评估PPAR-γ的表达。通过RT-PCR评估肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）或脂多糖（LPS）诱导的白细胞介素-8（IL-8）表达。
布拉酵母菌不影响HT-29细胞的活力和增殖。布拉酵母菌在mRNA和蛋白质水平上均上调PPAR-γ的表达。用布拉酵母菌预处理HT-29细胞可阻断TNF-α、IL-1β或LPS对PPAR-γ的下调作用，同时改善IL-8对这些促炎因子的反应。
布拉酵母菌刺激PPAR-γ表达并降低人结肠细胞对促炎细胞因子的反应。
