Lee Sang Kil, Kim Youn Wha, Chi Sung-Gil, Joo Yeong-Shil, Kim Hyo Jong
Department of Internal Medicine, Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Korea.
Dig Dis Sci. 2009 Feb;54(2):255-63. doi: 10.1007/s10620-008-0357-0. Epub 2008 Jul 10.
Saccharomyces boulardii (S. boulardii) has beneficial effects in the treatment of intestinal inflammation; however, little is known about the mechanisms by which these effects occur. We investigated the effects of S. boulardii on the expression of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) and interleukin-8 (IL-8), using human HT-29 colonocytes and a rat model of trinitrobenzene sulfonic acid (TNBS)-induced colitis. The effect of S. boulardii on gene expression was assessed by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and Northern blot and Western blot assays. Pharmacological inhibitors for various signaling pathways were used to determine the signaling pathways implicated in the S. boulardii regulation of PPAR-gamma and IL-8. We found that S. boulardii up-regulated and down-regulated PPAR-gamma and IL-8 expression at the transcription level, both in vitro and in vivo (P < 0.05, respectively). Saccharomyces boulardii blocked tumor necrosis factor-alpha (TNF-alpha) regulation of PPAR-gamma and IL-8 through disruption of TNF-alpha-mediated nuclear factor kappa B (NF-kappaB) activation. Furthermore, S. boulardii suppressed colitis and expression of pro-inflammatory cytokine genes in vivo (P < 0.05, respectively). Our study demonstrated that S. boulardii reduces colonic inflammation and regulates inflammatory gene expression.
布拉氏酵母菌（S. boulardii）在治疗肠道炎症方面具有有益作用；然而，对于这些作用产生的机制却知之甚少。我们使用人HT-29结肠细胞和三硝基苯磺酸（TNBS）诱导的大鼠结肠炎模型，研究了布拉氏酵母菌对过氧化物酶体增殖物激活受体γ（PPAR-γ）和白细胞介素-8（IL-8）表达的影响。通过半定量逆转录-聚合酶链反应（RT-PCR）、Northern印迹和Western印迹分析评估布拉氏酵母菌对基因表达的影响。使用各种信号通路的药理学抑制剂来确定参与布拉氏酵母菌对PPAR-γ和IL-8调节的信号通路。我们发现，布拉氏酵母菌在体外和体内均在转录水平上调和下调PPAR-γ和IL-8的表达（分别为P < 0.05）。布拉氏酵母菌通过破坏肿瘤坏死因子-α（TNF-α）介导的核因子κB（NF-κB）激活来阻断TNF-α对PPAR-γ和IL-8的调节。此外，布拉氏酵母菌在体内抑制结肠炎和促炎细胞因子基因的表达（分别为P < 0.05）。我们的研究表明，布拉氏酵母菌可减轻结肠炎症并调节炎症基因表达。
