Krause J R, Blank M K
Department of Pathology, University of Pittsburgh School of Medicine, Pennsylvania.
Anal Quant Cytol Histol. 1992 Apr;14(2):89-95.
DNA ploidy analysis was determined on 100 consecutive tumors from a wide variety of sites using both fresh and paraffin-embedded tissue on the same specimen. The correlation coefficient (r) value between the methods was 0.85. Aneuploidy was detected by both methods in 51/100 (51%) of the cases. Fresh tissue analysis yielded 10 additional cases (overall 61% aneuploidy) not detected on corresponding paraffin-embedded sections, whereas paraffin-embedded analysis detected 4 additional cases (overall 55% aneuploidy) not revealed by fresh tissue analysis. Fresh tissue analysis produced lower coefficients of variation and resulted in a cleaner preparation with less cellular debris. Fresh tissue analysis was also superior to paraffin for the detection of hypodiploid, near-diploid and multiple peaks. Analysis of paraffin-embedded material allows examination of archival tissue and provides a more rapid means of long-term follow-up and statistical correlations for prognostic studies. Although the overall correlation of both methodologies for DNA analysis showed a minimal variation in results, in our experience fresh tissue analysis has an advantage and is preferable, when available, for ploidy analysis.
使用同一标本上的新鲜组织和石蜡包埋组织，对来自各种部位的100例连续肿瘤进行了DNA倍体分析。两种方法之间的相关系数（r）值为0.85。两种方法在51/100（51%）的病例中均检测到非整倍体。新鲜组织分析发现了另外10例在相应石蜡包埋切片上未检测到的病例（总体非整倍体率为61%），而石蜡包埋分析检测到了另外4例新鲜组织分析未显示的病例（总体非整倍体率为55%）。新鲜组织分析产生的变异系数较低，并且制备更干净，细胞碎片更少。新鲜组织分析在检测亚二倍体、近二倍体和多个峰方面也优于石蜡。石蜡包埋材料的分析允许检查存档组织，并为长期随访和预后研究的统计相关性提供了一种更快的方法。尽管两种DNA分析方法的总体相关性显示结果差异最小，但根据我们的经验，新鲜组织分析具有优势，并且在可行时，对于倍体分析更可取。
