Paulsen Friedrich, Varoga Deike, Paulsen Andreas, Tsokos Michael
Department of Anatomy and Cell Biology, Martin Luther University of Halle-Wittenberg, Grosse Steinstrasse 52, 06097, Halle (Saale), Germany.
Cell Tissue Res. 2005 Jul;321(1):67-74. doi: 10.1007/s00441-005-1131-7. Epub 2005 May 21.
Vater's ampulla is of great clinical relevance with regard to the influx of chyme, ascending inflammation, intubation during diagnostic and therapeutic endoscopic maneuvers, therapeutic papillotomy and, especially, the formation of malignancies. Little is known about the distribution of trefoil factor family (TFF) peptides in the ampulla. We have therefore examined TFF peptide distribution in the normal ampulla of Vater and compared it with that in duodenal mucosa and Brunner's glands. Expression and synthesis of TFF peptides in Vater's ampulla and duodenum was investigated by reverse transcription-polymerase chain reaction, Western blot and immunohistochemistry. The samples studied originated from 30 autopsy cases with short postmortem intervals. TFF3 was expressed in the ampulla of Vater. mRNA expression of TFF1 was detected in only approximately 25% of the investigated samples. Western blot revealed the production of TFF3 and immunohistochemistry showed that TFF3 was the product of goblet cells. TFF peptide composition of Vater's ampulla varied in comparison with that in the duodenum regarding TFF2 expression. The ampulla of Vater thus has a unique profile of TFF peptide production, supporting the hypothesis that the ampulla is an autonomous organ. The observed differences in the TFF peptide distribution between the duodenum and Vater's ampulla favour the investigation of TFF peptides as prognostic markers in the classification of ampullary carcinomas.
壶腹在食糜涌入、炎症上行、诊断和治疗性内镜操作期间的插管、治疗性乳头切开术，尤其是恶性肿瘤形成方面具有重大临床意义。关于三叶因子家族（TFF）肽在壶腹中的分布情况，人们了解甚少。因此，我们研究了TFF肽在正常 Vater 壶腹中的分布，并将其与十二指肠黏膜和十二指肠腺中的分布进行比较。通过逆转录-聚合酶链反应、蛋白质印迹法和免疫组织化学研究了 Vater 壶腹和十二指肠中 TFF 肽的表达与合成。所研究的样本来自 30 例尸检病例，死后间隔时间较短。TFF3 在 Vater 壶腹中表达。仅在约 25% 的研究样本中检测到 TFF1 的 mRNA 表达。蛋白质印迹法显示有 TFF3 产生，免疫组织化学表明 TFF3 是杯状细胞的产物。就 TFF2 表达而言，Vater 壶腹的 TFF 肽组成与十二指肠中的不同。因此，Vater 壶腹具有独特的 TFF 肽产生特征，支持壶腹是一个自主器官的假说。十二指肠和 Vater 壶腹之间 TFF 肽分布的观察差异有利于将 TFF 肽作为壶腹癌分类中的预后标志物进行研究。
