Li Bin, Xie Guan-lin, Soad A, Coosemans J
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China.
J Zhejiang Univ Sci B. 2005 Jun;6(6):496-501. doi: 10.1631/jzus.2005.B0496.
Four rhizobacteria selected out of over 500 isolates from rhizosphere of the vegetables in China were further studied for suppression of the root-knot nematode and soil-borne fungal pathogens in laboratory and greenhouse in Belgium. They were identified as Brevibacillus brevis or Bacillus subtilis by Biolog test and partial 16s rDNA sequence comparison. They not only inhibited the radial growth of the root-infecting fungi Rhizoctonia solani SX-6, Pythium aphanidermatum ZJP-1 and Fusarium oxysporum f.sp. cucumerinum ZJF-2 in vitro, but also exhibited strong nematicidal activity by killing the second stage larvae of Meloidogyne javanica to varying degrees in the greenhouse. The toxic principles of bacterium B7 that showed the highest juvenile mortality were partially characterized. The active factors were heat stability and resistance to extreme pH values. B7 used either as seed dressing or soil drench significantly reduced the nematode populations in the rhizosphere and enhanced the growth of mungbean plants over the controls in the presence or absence of R. solani.
从中国蔬菜根际的500多个分离菌株中筛选出的4株根际细菌，在比利时的实验室和温室中进一步研究其对根结线虫和土传真菌病原体的抑制作用。通过Biolog测试和部分16s rDNA序列比较，它们被鉴定为短短芽孢杆菌或枯草芽孢杆菌。它们不仅在体外抑制了侵染根部的真菌立枯丝核菌SX-6、瓜果腐霉ZJP-1和尖孢镰刀菌黄瓜专化型ZJF-2的径向生长，而且在温室中通过不同程度地杀死爪哇根结线虫的二龄幼虫表现出很强的杀线虫活性。对显示最高幼虫死亡率的细菌B7的毒性原理进行了部分表征。活性因子具有热稳定性和对极端pH值的抗性。在有或没有立枯丝核菌的情况下，B7用作拌种剂或土壤浇灌剂均显著降低了根际线虫数量，并促进了绿豆植株的生长，优于对照。
