Islamic Azad University, Marvdasht Branch, Marvdasht, Fars, Iran.
J Invertebr Pathol. 2010 Jun;104(2):125-33. doi: 10.1016/j.jip.2010.03.002. Epub 2010 Mar 7.
Among fungi, species of the genus Pochonia Batista & O.M. Fonseca are considered as promising biological control agents with high potential to reduce root-knot nematode (RKN) and nematode populations. In this research we investigated Fars province of Iran for the presence of Pochonia spp., compared pathogenicity of different Pochonia species on eggs of RKN in vitro, and selected the best isolates for further studies. During 2004-2006, 128 soil samples of fields infested with cyst nematodes and 18 soil samples infested with RKN were collected from Fars province of Iran. In vitro pathogenicity tests were carried out on 36 isolates of Pochonia spp. obtained from CBS and IRAN culture collections. The seven best isolates of this experiment were selected for greenhouse test and their ability in controlling RKN was examined in natural soil. In greenhouse test fresh weight of plant's tops and roots, gall index, nematode multiplication, second-stage juveniles' population in soil, reproduction rate (P(f)/P(i)), proportion of infected eggs, control efficacy, root colonization and soil colony forming units were determined. In vitro pathogenicity of Pochonia on RKN eggs varied between 39% and 95% eggs infected. In greenhouse experiment, three isolates are promising for control of RKN and selected isolates are subjected to more extensive testing to determine their effectiveness in a range of conditions before being developed as commercial biological control agents.
在真菌中，波壳属（Pochonia）的物种被认为是有前途的生物防治剂，具有降低根结线虫（RKN）和线虫种群的高潜力。在这项研究中，我们调查了伊朗法尔斯省是否存在波壳属物种，比较了不同波壳属物种对 RKN 卵的体外致病性，并选择了最好的分离物进行进一步研究。2004-2006 年，从伊朗法尔斯省采集了 128 个感染胞囊线虫和 18 个感染 RKN 的田间土壤样本。从 CBS 和伊朗培养物收集获得了 36 个波壳属分离物，进行了体外致病性测试。从该实验中选择了 7 个最好的分离物进行温室试验，并在自然土壤中检查了它们控制 RKN 的能力。在温室试验中，测定了植物顶部和根部的鲜重、虫瘿指数、线虫繁殖、土壤中二龄幼虫种群、繁殖率（P(f)/P(i)）、感染卵比例、防治效果、根定殖和土壤集落形成单位。波壳属对 RKN 卵的体外致病性在 39%至 95%感染卵之间变化。在温室试验中，有三个分离物对 RKN 具有控制潜力，选择的分离物将进行更广泛的测试，以确定它们在一系列条件下的有效性，然后才能开发为商业生物防治剂。
