Bernard F X, Barrault C, Juchaux F, Laurensou C, Apert L
BIOalternatives SAS, Gençay, France.
J Wound Care. 2005 May;14(5):215-20. doi: 10.12968/jowc.2005.14.5.26775.
The effect of Urgotul on normal human dermal fibroblast proliferation was studied in vitro and compared with that of two other dressings: Mepitel and Tulle Gras.
Proliferation was measured by the extent of thymidine incorporation into the replicating DNA of proliferative fibroblasts in contact with the complete dressing.Additional cell viability and metabolism were evaluated using MTT assay. Morphology and ultrastructure analysis were based on immunolabelling and confocal laser microscopy.
Only Urgotul significantly stimulated thymidine incorporation, generally with a maximal proliferative effect at a contact time of 48 hours. This was confirmed by the observation of a greater number of dividing cells (mitotic cells) than in the control cultures. No cytotoxicity was observed following treatment with this dressing. Cells exhibited normal structural and ultrastructural features.
Fibroblasts play a key role in dermal wound repair. The ability of Urgotul to promote fibroblast proliferation could explain its efficiency in the healing process of acute and chronic wounds.
This study was supported by Urgo Laboratories.
在体外研究优拓SSD对正常人皮肤成纤维细胞增殖的影响，并与另外两种敷料：美皮康和优拓比较。
通过测量胸苷掺入与完整敷料接触的增殖性成纤维细胞复制DNA的程度来测定增殖情况。使用MTT法评估额外的细胞活力和代谢。形态学和超微结构分析基于免疫标记和共聚焦激光显微镜检查。
只有优拓SSD能显著刺激胸苷掺入，通常在接触48小时时具有最大增殖效应。观察到分裂细胞（有丝分裂细胞）数量比对照培养物中更多，证实了这一点。用这种敷料处理后未观察到细胞毒性。细胞表现出正常的结构和超微结构特征。
成纤维细胞在皮肤伤口修复中起关键作用。优拓SSD促进成纤维细胞增殖的能力可以解释其在急性和慢性伤口愈合过程中的有效性。
本研究由优格实验室资助。
