Molnár-Perl I, Füzfai Zs
Institute of Inorganic and Analytical Chemistry, L. Eötvös University, Budapest 112, H-1518, PO Box 32, Hungary.
J Chromatogr A. 2005 May 6;1073(1-2):201-27. doi: 10.1016/j.chroma.2004.10.068.
An overview is presented of chromatographic methods currently in use to determine flavonoids, including free aglycones, their corresponding glycosides, one by one, and, in the presence of each other. As a basis of selection, the following approaches can be distinguished: critical evaluation of the preliminary steps (extraction/isolation and hydrolysis) as well as the separation, identification and quantitation of constituents both on the basic research level and/or subsequently to various work up procedures. Chromatographic techniques were discussed after extraction/isolation of various flavonoids from several natural matrices. Papers were classified and compared from analytical point of view, primarily on the chromatographic, secondly on the detection techniques applied.
本文概述了目前用于测定黄酮类化合物的色谱方法，包括游离苷元、其相应的糖苷，以及它们同时存在时的逐一测定。作为选择的依据，可以区分以下方法：对初步步骤（提取/分离和水解）以及在基础研究层面和/或各种后处理程序之后对成分的分离、鉴定和定量进行批判性评估。在从几种天然基质中提取/分离出各种黄酮类化合物后，讨论了色谱技术。从分析角度对论文进行了分类和比较，主要基于色谱，其次基于所应用的检测技术。
