Harnly James M, Bhagwat Seema, Lin Long-Ze
US Department of Agriculture, Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, Beltsville, MD 20705, USA.
Anal Bioanal Chem. 2007 Sep;389(1):47-61. doi: 10.1007/s00216-007-1424-7. Epub 2007 Jul 25.
Profiling methods are needed that separate and detect all the phenolic compounds in a single extract of a food material. These methods must be comprehensive, rapid, and rich in spectral information. Fourteen methods that meet, or have the potential to meet, these criteria have been selected from the recent literature for review. In general, the methods employ a single aqueous methanol extraction, separation on a reversed-phase C column, and detection by UV/vis spectroscopy and mass spectrometry. The variations in extraction, separation, and detection are discussed. An increasingly important aspect of these methods is the archiving of data to permit cross-comparison of samples and standards and retrospective analysis. This review shows that the necessary technology is available to achieve the desired analytical goals.
需要有能够在食品原料的单一提取物中分离并检测所有酚类化合物的分析方法。这些方法必须具备全面性、快速性，且光谱信息丰富。从近期文献中挑选了十四种符合或有潜力符合这些标准的方法进行综述。总体而言，这些方法采用单一的甲醇水溶液萃取，在反相C柱上进行分离，并通过紫外/可见光谱和质谱进行检测。文中讨论了萃取、分离和检测方面的差异。这些方法中一个日益重要的方面是数据存档，以便对样品和标准进行交叉比较以及回顾性分析。这篇综述表明，现有的技术能够实现预期的分析目标。
