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南瓜中类胡萝卜素的提取与色谱分析

Extraction and chromatography of carotenoids from pumpkin.

作者信息

Seo Jung Sook, Burri Betty Jane C, Quan Zhejiu, Neidlinger Terry R

机构信息

Nutrition Department, Yuengnam University, Daegu, South Korea.

出版信息

J Chromatogr A. 2005 May 6;1073(1-2):371-5. doi: 10.1016/j.chroma.2004.10.044.

DOI:10.1016/j.chroma.2004.10.044
PMID:15909543
Abstract

Vitamin A deficiency is a health problem in Southeast Asia that can be corrected by feeding orange fruits and vegetables such as mango. Pumpkin is a traditional Korean food that is easy to store and is already believed to have health benefits. We extracted carotenoids from pumpkin by liquid-liquid extraction and by supercritical fluid extraction. We measured carotenoids by reversed-phase chromatography with diode array detection. The major carotenoid in pumpkin (> 80%) is beta-carotene, with lesser amounts of lutein, lycopene, alpha-carotene and cis-beta-carotene. Pumpkin is a rich source of beta-carotene and might be useful for preventing Vitamin A deficiency.

摘要

维生素A缺乏症是东南亚地区的一个健康问题,食用橙色水果和蔬菜(如芒果)可以纠正这一问题。南瓜是一种传统的韩国食物,易于储存,并且人们已经认为它对健康有益。我们通过液液萃取和超临界流体萃取从南瓜中提取类胡萝卜素。我们使用二极管阵列检测的反相色谱法测量类胡萝卜素。南瓜中的主要类胡萝卜素(>80%)是β-胡萝卜素,还有少量的叶黄素、番茄红素、α-胡萝卜素和顺式β-胡萝卜素。南瓜是β-胡萝卜素的丰富来源,可能有助于预防维生素A缺乏症。

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