Hong Liu, Peptan Ioana, Clark Paul, Mao Jeremy J
Department of Orthodontics, University of Illinois at Chicago, Chicago, IL 60612-7211, USA.
Ann Biomed Eng. 2005 Apr;33(4):511-7. doi: 10.1007/s10439-005-2510-7.
The limitation of current clinical treatment for restoration extended defects of soft tissue associated with trauma, tumor resections, and congenital deformities are well known. This study demonstrates that human bone marrow stromal cells (MSCs) can be utilized to tissue engineer adipose tissue for therapeutic purposes. Adipogenic potentials of monolayer-cultured human MSCs were evaluated by biochemical measurement of an adipogenic differentiation marker (glycerol-3-phosphate dehydrogenase, G-3-PDH) and cellular morphology. After preparation by seeding human MSCs on a 3-dimensional gelatin sponge and exposure to adipogenic differentiation medium, the ex vivo tissue-engineered adipose constructs were assessed histomorphologically and biochemically. Lipid droplets accumulated and expanded within the constructs accompanied by a significant increase of G-3-PDH activity. The present study indicates that bone MSCs could be a cell resource in tissue engineering adipose tissue, while gelatin sponge could be a good scaffold in this approach to improve the outcome of clinical treatment.
目前针对创伤、肿瘤切除及先天性畸形相关软组织大面积缺损的临床治疗局限性是众所周知的。本研究表明，人骨髓基质细胞（MSCs）可用于组织工程化脂肪组织以达到治疗目的。通过对脂肪生成分化标志物（甘油-3-磷酸脱氢酶，G-3-PDH）进行生化测定及观察细胞形态，评估单层培养的人骨髓基质细胞的脂肪生成潜能。将人骨髓基质细胞接种于三维明胶海绵并置于脂肪生成分化培养基中培养后，对体外组织工程化脂肪构建体进行组织形态学和生化评估。构建体内脂滴积聚并扩大，同时G-3-PDH活性显著增加。本研究表明，骨髓间充质干细胞可作为组织工程化脂肪组织的细胞来源，而明胶海绵可作为一种良好的支架，以此改善临床治疗效果。
